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Patients with renal cancer have a larger proportion of high-density blood monocytes with increased lucigenin-enhanced chemiluminescence

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Abstract

The production of oxygen metabolites is probably important in cancer cell killing. The production of the superoxide anion, O2 −, can be measured by lucigenin-enhanced chemiluminescence (Cl). Previous studies have shown that whole-blood lucigenin-enhanced Cl is increased in cancer patients and that this increase is related to blood monocyte activity. The present investigation confirmed these findings and showed that whole-blood lucigenin-enhanced Cl was elevated in 17 patients with renal cell adenocarcinoma (P<0.001). The activity of the monocytes was studied more in detail, whereby monocytes were separated into different populations based upon differences in densities, i.e., high-density and low-density monocytes. The cancer patients had a significantly larger proportion of high-density monocytes (P<0.05) than controls. The lucigenin-enhanced Cl of purified high-density monocytes in controls was significantly higher than that of low-density monocytes (P<0.01). The authors conclude that the increase in the lucigenin-enhanced Cl of whole blood observed in cancer patients may partly reflect the increased activity of a larger proportion of high-density monocytes in these patients.

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Authors and Affiliations

  1. Laboratory for Inflammation Research, Department of Clinical Chemistry, University Hospital, S-751 85, Uppsala, Sweden

    Agneta Trulson & Per Venge

  2. Department of Oncology, University Hospital, S-751 85, Uppsala, Sweden

    Sten Nilsson

  3. Department of Urology, University Hospital, S-751 85, Uppsala, Sweden

    Einar Brekkan

Authors
  1. Agneta Trulson
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  2. Sten Nilsson
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  3. Einar Brekkan
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  4. Per Venge
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Additional information

This study was supported by grants from the Medical Research Council of the Medical Faculty of Uppsala University and the Swedish Cancer Society.

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Trulson, A., Nilsson, S., Brekkan, E. et al. Patients with renal cancer have a larger proportion of high-density blood monocytes with increased lucigenin-enhanced chemiluminescence. Inflammation 18, 99–105 (1994). https://doi.org/10.1007/BF01534602

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