Abstract
We assessed the role of acentric chromosome fragments in gene amplification by using cell fusion techniques to introduce the fragmented chromosomes of a donor Chinese hamster ovary (CHO) cell line that contained the dihydrofolate reductase (dhfr)gene(s) into a CHO cell line deficient for dhfr.Chromosome fragments were successfully integrated into cells at a frequency of approximately 3%. Methotrexate-resistant variants arose much more frequently in two cell lines derived from these successful cell fusions than in wild-type CHO cells. The hybrid cell lines also amplified their dhfrgenes more readily than did the CHO cell line used as dhfrdonor.
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Hahn, P., Morgan, W.F. & Painter, R.B. The role of acentric chromosome fragments in gene amplification. Somat Cell Mol Genet 13, 597–608 (1987). https://doi.org/10.1007/BF01534480
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DOI: https://doi.org/10.1007/BF01534480