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Effect of carrageenan-induced granuloma on hepatic cytochrome P-450 isozymes in rats

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Abstract

Carrageenan-induced granuloma was used to study the apoprotein and RNA content, and catalytic activities of several cytochrome P-450 isozymes in liver. This model allowed discrimination between acute and chronic phases of experimental inflammation. The expression of most isozymes studied (CYP2D, CYP2E1, CYP3A1 and CYP4A) was reduced to 20% of the control level during the acute phase and partially recovered (30–60% of control group) during the chronic phase. CYP2B1 content was decreased to 65% of control during the acute and chronic phases of inflammation. RNA (CYP2B1 and CYP2E1) showed a strong depression during the acute phase and recovered during the chronic phase, without differences between isoenzymes. In most cases, there was a good correlation between the apoprotein content of isozymes and related activities. Our results show that the depletion of cytochrome P-450 induced by inflammation depends on the severity of the disease. Experimental inflammation equally affect the transcription of CYP2B1 and CYP2E1, so differences in apoprotein content and related activities between isozymes may due to differential posttranscriptional regulation.

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  1. Jordi Muntané

    Present address: Jordi Muntané Relat, Unidad Trasplante, Hospital Reina Sofía, Av Menendez Pidal s/n, 14004, Córdoba, España

Authors and Affiliations

  1. Faculty of Biology, Unite of Physiology Department of Biochemistry and Physiology, Av. Diagonal 645, 08028, Barcelona, Spain

    Jordi Muntané & M. Teresa Mitjavila

  2. Laboratory of Genetics and Biochemical Toxicology, Instituto di Mutagenesi e Differenziamento, CNR, Via Svezia 10, 56124, Pisa, Italy

    Vincenzo Longo & Pier G. Gervasi

  3. Department of Physiological Chemistry, Karolinska Institute, S-10401, Stockholm, Sweden

    Magnus Ingelman-Sundberg

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  1. Jordi Muntané
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  2. Vincenzo Longo
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  3. M. Teresa Mitjavila
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  4. Pier G. Gervasi
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  5. Magnus Ingelman-Sundberg
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At the time of the study, visiting scientists at the Department of Physiological Chemistry, Karolinska Institutet, S-10401 Stockholm, Sweden.

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Muntané, J., Longo, V., Mitjavila, M.T. et al. Effect of carrageenan-induced granuloma on hepatic cytochrome P-450 isozymes in rats. Inflammation 19, 143–156 (1995). https://doi.org/10.1007/BF01534458

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