Abstract
A multiple mutagen-sensitive mutant (XUM1) of mouse T-cell lymphoma line, L5178Y, is hypersensitive to ionizing radiation, ultraviolet (UV) light, and cross-linking agents (such as mitomycin C). The frequency of transfection for XUML cells after exposure to calcium phosphate-coprecipitated pSV2neo DNA was more than 104-fold less effective than that for Ltk−aprt− (LTA) cells. Other transfection methods (DEAE-dextran and polybrene-DMSO) were not effective for L5178Y and XUM1 cells. The transfection-proficient trait of LTA cells was demonstrated to be genetically dominant by examining the transfection frequency in hybrid clones constructed between XUM1 and LTA cells. To circumvent the problem with XUM1, the LTA genes necessary for transformation processes were introduced into XUM1 cells by constructing hybrids between XUM1 and LTA cells irradiated with X-rays which causes directional chromosome elimination for hybrid cells. Four of 194 hybrid clones tested were transfection-proficient and hypersensitive to UV (XL102, XL107, XL215, and XL216). All four clones were not hypersensitive to X-rays or mitomycin C. The frequencies of transfection for XL102 and XL216 were nearly the same level as that for LTA cells. The efficiency of transfection for XL107 and XL215 was 10 to 100-fold lower than that for LTA cells.
Similar content being viewed by others
Literature cited
Graham, F.L., and Van der Eb, A.J. (1973).Virology 52:456–467.
Wiegler, M., Silverstein, S., Lee, L.S., Pellicer, A., Cheng, Y., and Axel, R. (1977).Cell 11:223–232.
Westerveld, A., Hoeijmakers, J.H.J., Van Duin, M., J. de Wit, Odijk, H., Pastink, A., Wood, R.D., and Bootsma, D. (1984).Nature 310:425–429.
Shiomi, T., Hieda-Shiomi, N., Hama-Inaba, H., Ito, A., and Sato, K. (1987).Mutat. Res. 182:223–227.
Shiomi, T., Hieda-Shiomi, N., and Sato, K. (1982).Somat. Cell Genet. 8:329–345.
Sato, K., And Setlow, R.B. (1981).Mutat. Res. 84:443–445.
Hori, T., Shiomi, T., and Sato, K. (1983).Proc. Natl. Acad. Sci. U.S.A. 80:5655–5659.
Sompayrac, L.M., and Danna, K.J. (1981).Proc. Natl. Acad. Sci. U.S.A. 78: 7575–7578.
Chaney, W.G., Howard, D.R., Pollard, J.W., Sallustio, S., and Stanley, P. (1986).Somat. Cell Mol. Genet. 12:237–244.
Kawai, S., and Nishizawa, M. (1984).Mol. Cell. Biol. 4:1172–1174.
Pontecorvo, G. (1971).Nature 230:367–369.
Chapman, V.M., and Shows, T.B. (1976).Nature 259:665–667.
Shiomi, T., Hieda-Shiomi, N., and Sato, K. (1983).Mutat. Res. 108:395–404.
Shiomi, T., Hieda-Shiomi, N., and Sato, K. (1982).Mutat. Res. 103:61–69.
Southern, P.J., and Berg, P. (1982).Mol. Appl. Genet. 1:327–341.
Maniatis, T., Fritsch, E.F., and Sambrook, J. (1982).Molecular Cloning: A Laboratory Manual, (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
Hama-Inaba, H., Hieda-Shiomi, N., Shiomi, T., and Sato, K. (1983).Mutat. Res. 108:405–416.
Sato, K., and Hieda, N. (1979).Int. J. Radiat. Biol. 35:83–87.
Tsuji, H., Shiomi, T., and Tobari, I. (1984). InSister Chromatid Exchange: 25 Years of Experimental Research, (eds.) Tice, R.R., and Hollaender, A. (Plenum Press, New York), pp. 109–126.
Van Duin, M., J. de Wit, Odijk, H., Westerveld, A., Yasui, A., Koken, M.H.M., Hoeijmakers, J.H.J., and Bootsma, D. (1986).Cell 44:913–923.
Jolly, D.J., Esty, A.C., Bernard, H.U., and Friedmann, T. (1982).Proc. Natl. Acad. Sci. U.S.A. 79:5038–5041.
Kai, R., Ohtsubo, M., Sekiguchi, M., and Nishimoto, T. (1986).Mol. Cell. Biol. 6:2027–2032.
Kuhn, L.C., MacClelland, A., and Ruddle, F.H., (1984).Cell 37:95–103.
Lowy, I., Pellicer, A., Jackson, J.F., Sim, G.K., Silverstein, S., and Axel, R. (1980)Cell 22:817–823.
Perucho, M., Hanahan, D., Lipsich L., and Wigler, M. (1980).Nature 285:207–210.
Takeishi, K., Ayusawa, D., Kaneda, S., Shimizu, K., and Seno, T. (1984).J. Biochem. 95:1477–1483.
Oi, V.T., Morrieon, S.L., Herzenberg, L.A., and Berg, P. (1983).Proc. Natl. Acad. Sci. U.S.A. 80:825–829.
Corsaro, C.M., and Pearson, M.L. (1981).Somat. Cell Genet. 7:617–630.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shiomi, T., Hieda-Shiomi, N., Sato, K. et al. Cell fusion-mediated improvement in transfection competence for repair-deficient mutant of mouse T cell line. Somat Cell Mol Genet 14, 195–203 (1988). https://doi.org/10.1007/BF01534404
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01534404