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In vitro evaluation ofPyrularia thionin—anti-CD5 immunotoxin

  • Original Article
  • Immunotoxin, Anti-CD5 Monoclonal Antibody, Membrane-Active Toxin, Pyrularia Thionin, T Lymphocytes, Cytotoxicity
  • Published:
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Abstract

The membrane-active peptide,Pyrularia thionin, purified fromPyrularia pubera, was covalently conjugated to an anti-CD5 monoclonal antibody. The membrane-active properties of thionin were not affected by the conjugation. The immunotoxin killed CD5+ lymphocytes in vitro at a concentration of 0.1 nmol/107 cells after 2 h of incubation. The immunotoxin also inhibited the proliferation of T cells in vitro, stimulated either by mitogens or in the mixed lymphocyte reaction. It was shown by electron paramagnetic resonance of spin probes and differential scanning calorimetry that the ability of the immunotoxin to perturb the lipid phase of membranes is close to that of unconjugated thionin. The results obtained suggest thatPyrularia-thionin—anti-CD5 conjugate may be useful for graft-versus-host disease therapy and potentially in the treatment of CD5+ leukemia and lymphomas.

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Authors and Affiliations

  1. Department of Biological Sciences, University of Texas at El Paso, 500 West University Avenue, 79968, El Paso, Texas, USA

    S. E. Gasanov & E. D. Rael

  2. Institute of Bioorganic Chemistry, Uzbek Academy of Sciences, 700143, Tashkent, Uzbekistan

    N. E. Gasanov

  3. Chemistry Department, Brigham Young University, 84602, Provo, Utah, USA

    L. P. Vernon

Authors
  1. S. E. Gasanov
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  2. E. D. Rael
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  3. N. E. Gasanov
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  4. L. P. Vernon
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Gasanov, S.E., Rael, E.D., Gasanov, N.E. et al. In vitro evaluation ofPyrularia thionin—anti-CD5 immunotoxin. Cancer Immunol Immunother 41, 122–128 (1995). https://doi.org/10.1007/BF01527408

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  • DOI: https://doi.org/10.1007/BF01527408

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