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Increase in the ability of human cancer cells to induce cytotoxic T lymphocytes by ultraviolet irradiation

  • Original Articles
  • Human Cancer Cells, Immunogenicity, Ultraviolet-B Irradiation, Cytotoxic T Lymphocytes
  • Published:
Cancer Immunology, Immunotherapy Aims and scope Submit manuscript

Abstract

The roles of ultraviolet-B (UV) radiation in the immunogenicity of human cancer cells have not been fully studied. We have investigated the effects of UV radiation on metastatic melanoma and renal cell carcinoma cells with regard to MHC antigen expression and the ability to induce cytotoxic T lymphocyte (CTL) activity in peripheral blood mononuclear cells (PBMC) or tumor-infiltrating lymphocytes (TIL) against untreated autologous tumor cells. UV radiation respectively decreased or increased MHC class I expression of freshly isolated tumor cells or cultured tumor cells, and also decreased MHC class I expression of starved cultured tumor cells. It increased the ability of both freshly isolated and cultured tumor cells to induce CTL activity from PBMC against untreated autologous tumor cells. UV-irradiated subclones that were more susceptible to CTL lysis were more potent for CTL induction from TIL than either an untreated parental clone or a UV-irradiated subclone that was resistant to CTL lysis. In summary, UV radiation increased the ability of tumor cells to induce CTL activity without a corresponding effect on MHC antigen expression.

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Authors and Affiliations

  1. Department of Immunology, University of Texas, M.D. Anderson Cancer Center, Houston, Texas, USA

    Yasuiki Umezu, Lazel B Augustus, Dai Seito, Kazuhiro Hayakawa & Kyogo Itoh

  2. Department of General Surgery, University of Texas, M.D. Anderson Cancer Center, Houston, Texas, USA

    Merric I Ross

  3. Department of Medical Oncology, University of Texas, M.D. Anderson Cancer Center, Houston, Texas, USA

    Omar Eton

  4. Department of Urology, University of Texas, M.D. Anderson Cancer Center, Houston, Texas, USA

    David A Swanson

  5. Department of Immunology, Kurume University School of Medicine, 67 Asahi-machi Kurume, 830, Kurume, Japan

    Kyogo Itoh

Authors
  1. Yasuiki Umezu
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  2. Lazel B Augustus
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  3. Dai Seito
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  4. Kazuhiro Hayakawa
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  5. Merric I Ross
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  6. Omar Eton
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  7. David A Swanson
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  8. Kyogo Itoh
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Additional information

This work was supported in part by a grant CA47891 from the National Cancer Institute, USA, a grant-in-aid of the comprehensive 10-years strategy for cancer control from ministry of a Health and Welfare, Japan, and the Ishibashi Research Fund, Japan

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Umezu, Y., Augustus, L.B., Seito, D. et al. Increase in the ability of human cancer cells to induce cytotoxic T lymphocytes by ultraviolet irradiation. Cancer Immunol Immunother 37, 392–399 (1993). https://doi.org/10.1007/BF01526796

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  • DOI: https://doi.org/10.1007/BF01526796

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