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Biological response modifiers (BRM) as antigens

III. T cell lines specific for BRM kill tumor cells in a BRM-specific manner

  • Original Article
  • Biological Response Modifier, T Cell Lines
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Abstract

In order to investigate tumoricidal effector cells in therapy by biological response modifiers (BRM) such asPropionibacterium acnes, bacillus Calmette-Guérin (BCG),Streptococcus pyogenes and a protein-bound polysaccharide (PSK), we established T cell lines specific for each BRM from BALB/c mice immunized with the corresponding BRM. These T cell lines proliferated and produced interleukin-2-(IL-2) and/or IL-4, but only in the presence of the relevant BRM and BALB/c spleen cells as the antigen and antigen-presenting cells respectively. Cross-functional experiments indicated that each BRM acts as a nominal antigen, but not as a non-specific immunostimulator. In addition, the T cell lines killed Ia-positive syngeneic B lymphoma cells, but only in the presence of the relevant BRM. These experiments excluded the possibility of cytotoxic effects by each BRM. The T cell lines and clones also killed Ia-negative bystander target cells, but only in the presence of both a relevant antigen and antigen-presenting cells. The T cell clones specific forS. pyogenes orP. acnes tested were Thy1+, L3T4+ and Lyt2−. These results indicate that some BRM exert tumoricidal activity by inducing T cells that recognize them as an antigen and kill tumor cells in an antigen-specific manner. The T cells killed tumor targets in either a tumor-necrosis-factor(TNF)-dependent or a TNF-independent manner. The mediator of the latter pathway remains to be elucidated.

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Authors and Affiliations

  1. The Second Division of Internal Medicine, Kyoto University Faculty of Medicine, 54 Shogoin-Kawahara-cho, Sakyo-ku, 606-01, Kyoto, Japan

    Shoichi Ozaki, Takahiro Okazaki & Kazuwa Nakao

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  1. Shoichi Ozaki
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  2. Takahiro Okazaki
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  3. Kazuwa Nakao
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Ozaki, S., Okazaki, T. & Nakao, K. Biological response modifiers (BRM) as antigens. Cancer Immunol Immunother 40, 219–227 (1995). https://doi.org/10.1007/BF01519895

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  • DOI: https://doi.org/10.1007/BF01519895

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