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Cytotoxic cell function and phenotypic analysis of peripheral blood mononuclear cells in cancer patients treated with low-dose interleukin-2 and mitomycin C

  • Original Articles
  • Cytotoxic Activity, Peripheral Blood Mononuclear Cell, Interleukin-2, Mitomycin C, Clinical Response
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Cancer Immunology, Immunotherapy Aims and scope Submit manuscript

Abstract

We previously found that the ability of peripheral blood mononuclear cells (PBM) of cancer patients to generate lymphokine-activated killer (LAK) cells became remarkably augmented after mitomycin C administration. On the basis of the clinical finding, we designed a treatment regimen comprised of 12 mg/m2 mitomycin C i. v. on day 1 and 700 U/m2 recombinant interleukin-2 (IL-2) i.v. every 12 h from day 4 through day 8. Of 25 patients with advanced carcinoma, 9 had a partial response and 3 had a minor response. Cytotoxic cell function, including natural killer activity, lymphokine-activated killer (LAK) activity, and the ability to generate LAK cells, and lymphocyte subsets in PBM was measured 1 day before and after either the first or second course of this therapy. The relationship between these parameters and the clinical antitumor response to this treatment was examined. Although the cytotoxic activities were significantly augmented after either the first or second treatment course, no positive correlation was observed between the changes in these cytotoxic activities and the clinical response to this therapy, when patients who either showed a partial response or whose disease remission was partial or minor were defined as responders. Further, phenotypic analysis showed a significant increase in CD2+, CD3+ CD4+ and CD4+Leu8− cells after the firs course, and CD25+ cells after either the first or second course of this treatment. The precentages of CD2+ and CD25+ cells were significantly elevated only in responders but not in nonresponders, suggesting the increase in these subsets was related to clinical response.

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Authors and Affiliations

  1. Department of Surgery, Medical Institute of Bioregulation, Kyushu University, 874, Beppu, Japan

    Shinya Arinaga, Nobuya Karimine, Masashi Adachi, Hiroshi Inoue, Shigeru Nanbara, Tsukasa Asoh, Hiroaki Ueo & Tsuyoshi Akiyoshi

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  1. Shinya Arinaga
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  2. Nobuya Karimine
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  3. Masashi Adachi
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  4. Hiroshi Inoue
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  5. Shigeru Nanbara
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  6. Tsukasa Asoh
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  7. Hiroaki Ueo
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  8. Tsuyoshi Akiyoshi
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Arinaga, S., Karimine, N., Adachi, M. et al. Cytotoxic cell function and phenotypic analysis of peripheral blood mononuclear cells in cancer patients treated with low-dose interleukin-2 and mitomycin C. Cancer Immunol Immunother 37, 220–226 (1993). https://doi.org/10.1007/BF01518514

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  • DOI: https://doi.org/10.1007/BF01518514

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