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On the locus of action of Na+ at site I of oxidative phosphorylation

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Abstract

The effect of octylguanidine on the Na+ stimulated oxygen uptake of rat liver mitochondria and bovine heart submitochondrial particles and on the Na+ induced efflux of K from the mitochondria has been examined. The results indicate that the action of Na+ is inhibited by octylguanidine, but that the degree of inhibition depends on the concentration of the cation. Apparently, a competition exists between Na+ and octylguanidine for a common site. Octylguanidine, but not oligomycin, at certain concentrations restores in mitochondria incubated with Na+ the capacity to respond to uncouplers. A competitive effect between monovalent cations and octylguanidine has been observed in submitochondrial particles oxidizing NADH.

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Authors and Affiliations

  1. Departamento de Bioquímica, Facultad de Medicina, Ciudad Universitaria, México, D.f., México

    A. Gómez-Puyou, F. Sandoval, M. Tuena De Gómez-Puyou, E. Chávez & E. Pinto

Authors
  1. A. Gómez-Puyou
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  2. F. Sandoval
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  3. M. Tuena De Gómez-Puyou
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  4. E. Chávez
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  5. E. Pinto
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Fellow of the Academia Nacional de la Investigación Científica and the Consejo Nacional de Ciencias y Tecnología

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Gómez-Puyou, A., Sandoval, F., De Gómez-Puyou, M.T. et al. On the locus of action of Na+ at site I of oxidative phosphorylation. J Bioenerg Biomembr 3, 221–233 (1972). https://doi.org/10.1007/BF01515970

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  • DOI: https://doi.org/10.1007/BF01515970

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