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The determination of work of compression of protein monolayers at the air-water interface

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Summary

In the present study, monolayers of various proteins were investigated at the air-water interface. The work of compression,W c (Helmholtz free energy) has been determined from the surface pressure-area compression isotherms. A linear relationship was found betweenW c and the amount of protein present at the surface. Further, it is shown that this relation holds both for completely unfolded (Bovine-serum-albumin, Ovalbumin) and for incompletely unfolded (Transferrin, Myoglobin) proteins.β-Lipoprotein isotherms also gave a similar dependence. It is further shown that the amount of protein lost into the subphase can be determined from a plot ofW c versus protein added at the interface. The results are discussed in relation to the constitution of protein molecules at the surface.

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Author notes

  1. K. S. Birdi

    Present address: Institute of Physical Chemistry, The Technical University of Denmark, Lyngby, Denmark

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    Birdi, K.S. The determination of work of compression of protein monolayers at the air-water interface. Kolloid-Z.u.Z.Polymere 250, 222–226 (1972). https://doi.org/10.1007/BF01507606

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    • DOI: https://doi.org/10.1007/BF01507606

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