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Genetic fine structure and polarized negative interference at thelys-51(FL) locus ofAspergillus nidulans

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Abstract

Twelve mutants at thelys-51(FL) locus ofAspergillus nidulans were intercrossed, and with the help of outside markers their relative order and approximate distances were mapped. Three distinct clusters were recognized A, B and C, where A is proximal to the centromere, C distal, and B centrally located. Recombination between members of cluster A and B, or B and C showed a characteristic pattern termed polarized negative interference (Siddiqi & Putrament, 1963). In addition, it was found that there is reversal of polarization within the locus (Pees, 1965), and that, with respect to the direction of polarization, there is a sharp “turning-point” at, or close to the mutantlys-12.21 within the B cluster. When any two mutants on the same side of this point and proximally to the centromere were crossed, negative interference was most intense in the proximal adjacent interval. When both mutants were beyond the turning-point, negative interference was most intense in the distal adjacent interval and always independent of recombination distances. When, however, the mutants were on either side of this turning-point, results were such as to suggest interference between the two opposed polarity patterns, their respective contributions to the final result being approximately proportional to the recombination distances between each mutant andlys-12.21.

These results were interpreted in terms of current gene recombination theory.

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Author notes

  1. Elisabeth Pees

    Present address: Department of Radiation Genetics, University of Leiden, Wassenaarseweg 62, Leiden, The Netherlands

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  1. Genetical Institute, University of Leiden, The Netherlands

    Elisabeth Pees

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  1. Elisabeth Pees
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This work forms part of a Thesis submitted in fulfillment of the requirements for a Ph. D. degree at the University of Leiden (1966).

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Pees, E. Genetic fine structure and polarized negative interference at thelys-51(FL) locus ofAspergillus nidulans . Genetica 38, 275–304 (1967). https://doi.org/10.1007/BF01507463

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  • DOI: https://doi.org/10.1007/BF01507463

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