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A vegetative instability inAspergillus nidulans

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Abstract

A proportion of segregants from a cross between strainsbi 1w3s5 andy pyro 4s12 were found to be unstable. The sectors to which they gave rise during vegetative growth, made up about 30% of all segregants from such crosses. Only thes 12 alleles appeared to be genetically affected. From the pattern of inheritance, and the range of sectors observed, the probable cause of the instability is a chromosomal aberration.

p-fluorophenylalanine, which is used to haploidise diploid strains, induces potentially unstable segregants to sector with great efficiency. Since the instability is probably due to an aberration, this amino acid analogue must also be an inducer, either of aberrations or, under certain circumstances, recombination.

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References

  • Bainbridge, B. W. &J. A. Roper (1966). Observations on the effects of a chromosome duplication inAspergillus nidulans.J. gen. Microbiol. 42: 417–424.

    PubMed  Google Scholar 

  • Barratt, R. W., G. B. Johnson &W. N. Ogata (1965). Wild type and mutant stocks ofAspergillus nidulans.Genetics 52: 233–246.

    PubMed  Google Scholar 

  • Catcheside, D. G. (1947). The P-locus position effect inOenothera.Journal Genetics 48: 31–42.

    Google Scholar 

  • Cox, B. S. &E. A. Bevan (1962). Aneuploidy in Yeast.New Phytol. 61: 342–355.

    Google Scholar 

  • Hussey, C., B. A. Orsi, J. Scott &B. Spencer (1965). Mechanism of Choline sulphate utilization in fungi.Nature 207: 632–633.

    Google Scholar 

  • Käfer, E. (1958). An 8-chromosome map ofAspergillus nidulans.Advances in Genetics 9: 105–145.

    PubMed  Google Scholar 

  • Käfer, E. (1960). High frequence of spontaneous and induced somatic segregation inAspergillus nidulans.Nature 186: 619.

    Google Scholar 

  • Käfer, E. (1961). The process of spontaneous recombination in vegetative nuclei ofAspergillus nidulans.Genetics 46: 1581–1609.

    PubMed  Google Scholar 

  • Käfer, E. (1962). Translocations in stock strains ofAspergillus nidulans.Genetica 33: 59–68.

    Google Scholar 

  • Käfer, E. (1965). Origins of translocations inAspergillus nidulans.Genetics 52: 217–232.

    PubMed  Google Scholar 

  • Käfer-Boothroyd, E. (1965). Tests for translocations by mitotic linkage in heterozygous diploids.Aspergillus News Letter 6: 21–22.

    Google Scholar 

  • Lewis, E. B. (1950). The phenomenon of position effect.Adv. in Genetics 3: 73–115.

    Google Scholar 

  • Lhoas, P. J. (1961). Mitotic haploidisation by treatment ofAspergillus niger diploids withpara-fluoro-phenylalanine.Nature 190: 744.

    PubMed  Google Scholar 

  • McClintock, B. (1941). The stability of broken ends of chromosomes inZea mays.Genetics 26: 234–282.

    Google Scholar 

  • McClintock, B. (1945). Neurospora. I. preliminary observations of the chromosomes ofNeurospora crassa.Amer. J. Bot. 32: 671–678.

    Google Scholar 

  • McCully, K. S. &E. Forbes (1965). The use ofp-fluorophenylalanine with “master strains” ofAspergillus nidulans for assigning genes to linkage groups.Genet. Res., Camb. 6: 352–359.

    Google Scholar 

  • Morpurgo, G. (1961). Somatic segregation induced byp-fluoro-phenylalanine.Aspergillus News Letter 2: 10.

    Google Scholar 

  • Morpurgo, G. (1963). Somatic segregation induced byp-fluorophenylalanine.Aspergillus News Letter 4: 8.

    Google Scholar 

  • Nga, B. H. & J. A. Roper (1966). A chromosomal process giving rise to vegetative instability inAspergillus.Com. to the Genet. Soc. 151.

  • Orsi, B. A. &B. Spencer (1964). Choline sulphokinase.J. Biochem. 56: 81–91.

    PubMed  Google Scholar 

  • Pontecorvo, G., J. A. Roper, L. M. Hemmons, K. D. MacDonald &A. W. J. Bufton (1953). The Genetics ofAspergillus nidulans.Adv. in Genet. 5: 141–238.

    Google Scholar 

  • Roper, J. A. (1966). Culture temperature and biotin requirement inAspergillus.Aspergillus News Letter 7: 22.

    Google Scholar 

  • Schmidt, E. &W. Wagner (1904). Über Cholin, Lezithin und verwandte Verbindungen.Ann. Chem. 337: 51.

    Google Scholar 

  • Spencer, B. &T. Harada (1960). The role of choline sulphate in the sulphate metabolism of fungi.Biochem. J. 77: 305–315.

    Google Scholar 

  • Strigini, P. &G. Morpurgo (1961) Biotin requirement by wild type Aspergillus nidulans andNeurospora crassa.Aspergillus New Letter 2: 9.

    Google Scholar 

  • Swanson, C. P. (1960). Cytology and Cytogenetics. MacMillan & Co., London.

    Google Scholar 

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Authors and Affiliations

  1. Genetisch Laboratorium der Rijksuniversiteit, Kaiserstraat 63, Leiden, The Netherlands

    A. M. Millington-Ward

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  1. A. M. Millington-Ward
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This work was completed at Trinity College, Dublin in the Summer of 1966.

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Millington-Ward, A.M. A vegetative instability inAspergillus nidulans . Genetica 38, 191–207 (1967). https://doi.org/10.1007/BF01507458

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  • DOI: https://doi.org/10.1007/BF01507458

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