Zusammenfassung
Plasmazytoide Zellen im Knochenmark von drei Patienten mit Myelom und Plasmazellen im Knochenmark eines sechsjährigen Knaben mit Infekt wurden zuerst nach Giemsafärbung und anschließend nach Feulgenfärbung zytophotometrisch gemessen. An Hand der Anzahl Meßpunkte konnte die Zellfläche und die Kernfläche und daraus die Kern-Plasma-Relation bestimmt werden. Die mittlere Kern-Plasma-Relation und der mittlere DNS-Verteilungsfehler im Kern variieren von Myelompatient zu Myelompatient und erlaubten nicht, Plasmazellen von Myelomzellen zu unterscheiden. Eine Differenzierung dieser Zelltypen war auch auf Grund des Produktes aus mittlerer Kern-Plasma-Relation und mittlerem DNS-Verteilungsfehler nicht möglich. Zwischen Zellfläche und Zytoplasmafläche bestand eine signifikante Korrelation sowohl bei den Myelomzellen als auch bei den Plasmazellen.
Summary
Plasmacytoid cells in the bone marrow of 3 patients with myeloma and plasma cells in the bone marrow of a 6-year-old boy with an infectious disease were assessed cytophotometrically, first after Giemsa-and second after Feulgen staining. The cell and nuclear surface and the nuclear/cytoplasmic ratio were determined from the number of measuring points. The nuclear DNA content of individual cells was registered and the distribution of DNA within the nucleus was assessed by the distributional error. Both the mean nuclear/cytoplasmic ratio and the distributional error of myeloma cells varied from patient to patient but could not be used to differentiate between normal plasma cells and myeloma cells. It was not possible either to differentiate these cell types by multiplying the mean nuclear/cytoplasmic ratio with the mean distributional error of the nuclear DNA. A strong correlation between cell and cytoplasmic surface area was observed both in normal plasma cells and in myeloma cells.
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Supported by the Swiss National Foundation for Scientific Research and by the Swiss Cancer League
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Kühner, U., Barandun, S., Morell, A. et al. Differentiation of plasma and myeloma cells of man combined planimetric and cytophotometric studies. Klin Wochenschr 55, 573–576 (1977). https://doi.org/10.1007/BF01490510
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DOI: https://doi.org/10.1007/BF01490510