Summary
In studying the incorporation of the purine precursor glycine-1-C14 into the adenine nucleotides of human and pig lymphocytesin vitro no labelling of adenine nucleotides could be found. It is therefore concluded that mature circulating lymphocytes do not synthesize purinesde novo. However, by stimulating the lymphocytes with PHA, induction of thede novo synthesis of purine bases could be observed. Kinetic studies of this process show thatde novo synthesis is not detectable within the first 2 hours after the addition of PHA to the lymphocyte culture, but that it increases rapidly in the following hours.
Zusammenfassung
Weder in menschlichen noch in Schweine-Lymphocyten ist nachin vitro Inkorporation mit 1-C14-Glycin eine Markierung freier Adeninnucleotide nachzuweisen. Dies läßt den Schluß zu, daß Lymphocyten des zirkulierenden Blutes unter den angegebenen experimentellen Bedingungen nicht oder nur in unzureichendem Maße befähigt sind, Purinnucleotidede novo zu synthetisieren. Werden die Lymphocyten dagegen mit PHA stimuliert, so kommt es zur Induktion derde novo Synthese von Adeninnucleotiden. Kinetische Studien dieses Prozesses lassen erkennen, daß während der ersten 2 Std nach Zugabe des PHA zur Lymphocytenkultur noch kein, in den folgenden Stunden jedoch ein rascher Anstieg der Synthesevorgänge eintritt.
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Mit Unterstützung des Fonds zur Förderung der wissenschaftlichen Forschung (Österreich).
Auszugsweise vorgetragen während des Ist Meeting Europ. Div. Int. Soc. Haematology, Mailand, Sept. 1971.
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Schwarzmeier, J., Moser, K. & Rainer, H. De novo Synthese von Adeninnucleotiden in Lymphocyten: Induktion durch Phytohämagglutinin. Klin Wochenschr 50, 871–874 (1972). https://doi.org/10.1007/BF01488944
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DOI: https://doi.org/10.1007/BF01488944