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Inhibition of rat pleural mesothelial cell nitric oxide synthesis by transforming growth factor-β1

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Abstract

Pleuritis is a common initial clinical manifestation of tuberculosis. It is associated with an accumulation of a variety of cytokines in the pleura and pleural fluid. We have recently shown that these proinflammatory cytokines induce the pleural mesothelial cell to produce large amounts of nitric oxide, a nitrogen intermediate that has been shown to have a tuberculocidal effect. TGF-β has also been found in situ in tuberculous effusions and pleural tissues and is thought to suppress the immune response and promote tissue repair. This study examined the effects of TGF-β on cytokine-induced NO synthesis by rat pleural mesothelial cells in vitro. Results demonstrated that TGF-β significantly inhibited NO synthesis and that this inhibition was associated with a proportionate decrease in iNOS mRNA and iNOS protein. Suppression of pleural mesothelial cell NO synthesis by TGF-β may be important in the pathogenesis of tuberculous pleuritis.

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Authors and Affiliations

  1. Departments of Medicine and Physiology and Biophysics, Louisiana State University, USA

    Matthew B. Grisham

  2. Overton Brooks Veterans Affairs Medical Centers, Shreveport, Louisiana

    Michael W. Owens & Shawn A. Milligan

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  1. Michael W. Owens
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  2. Shawn A. Milligan
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  3. Matthew B. Grisham
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Owens, M.W., Milligan, S.A. & Grisham, M.B. Inhibition of rat pleural mesothelial cell nitric oxide synthesis by transforming growth factor-β1. Inflammation 20, 637–646 (1996). https://doi.org/10.1007/BF01488801

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