Skip to main content
SpringerLink
Log in

Endothelial cell associated anti-elastolytic activity

  • Original Articles
  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

Addition of cultured and then carefully-washed bovine pulmonary artery endothelial cells (EC) decreased (p < 0.05) human neutrophil elastase activity (HNE) in vitro. HNE activity was also decreased (p < 0.05) by addition of histone or protamine treated EC. However, addition of papain or trypsin treated EC decreased HNE activity less than addition of untreated cells suggesting that a protein rather than a difference in cell surface charge was responsible. Other observations suggest that EC anti-elastolytic activity was not due to binding of antiprotease from culture media but was dependent on EC protein synthesis. First, addition of EC grown previously in serum-free media decreased HNE activity the same (p < 0.05) as addition of EC cultured in media containing serum. Second, addition of EC treated beforehand with cycloheximide decreased HNE activity less than (p < 0.05) addition of untreated control EC. We conclude that EC most likely make and have antielastolytic activity on their surfaces and speculate that EC associated anti-elastolytic activity may modulate inflammatory, repair and other biologic processes involving neutrophil elastase.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Janoff, A. 1985. Elastases and emphysema: current assessment of the protease-antiprotease hypothesis.Am. Rev. Respir. Dis. 132:417–433.

    Google Scholar 

  2. Carp, H., andA. Janoff. 1978. Possible mechanisms of emphysema in smokers. In vitro suppression of serum-inhibitory capacity by fresh cigarette smoke and its prevention by antioxidants.Am. Rev. Respir. Dis. 118:617–621.

    Google Scholar 

  3. Abboud, R. T., T. Fera, A. Richter, M. Z. Tabona, andS. Johal. 1985. Acute effect of smoking on the functional activity of alpha1-protease inhibitor in bronchoalveolar lavage fluid.Am. Rev. Respir. Dis. 131:79–85.

    Google Scholar 

  4. Carp, H., F. Miller, J. R. Hoidal, andA. Janoff. 1982. Alpha1-proteinase inhibitor purified from lungs of cigarette smokers contains oxidized methionine and has decreased elastase inhibitory capacity.Proc. Natl. Acad. Sci. USA 79:2041–2045.

    Google Scholar 

  5. Laurell, C. B., andS. Eriksson. 1963. The electrophoretic alpha1-globulin pattern of serum in alpha1-antitrypsin deficiency.Scand. J. Clin. and Lab. Invest. 15:132–140.

    Google Scholar 

  6. Abrams, W. R., A. B. Cohen, V. V. Damiano, A. Eliraz, P. Kimbel, D. R. Meranze, andG. Weinbaum. 1981. A model of decreased functional alpha-1-proteinase inhibitor. Pulmonary pathology of dogs exposed to chloramine T.J. Clin. Invest. 68:1132–1139.

    Google Scholar 

  7. Abrams, W. R., A. Eliraz, P. Kimbel, andG. Weinbaum. 1980. The effect of the oxidizing agent chloramine T and cigarette smoke on dog serum proteinase inhibitors.Exp. Lung Res. 1:211–223.

    Google Scholar 

  8. Bachofen, M., andE. R. Weibel. 1977. Alterations of the gas exchange apparatus in adult respiratory insufficiency associated with septicemia.Am. Rev. Respir. Dis. 116:589–615.

    Google Scholar 

  9. Weiland, J. E., W. B. Davis, J. R. Holter, J. R. Mohammed, P. M. Dorinsky, andJ. E. Gadek. 1986. Lung neutrophils in the adult respiratory distress syndrome: Clinical and pathophysiologic significance.Am. Rev. Respir. Dis. 133:218–225.

    Google Scholar 

  10. Cochrane, C. G., R. Spragg, andS. D. Revak. 1983. Pathogenesis of the adult respiratory distress syndrome: evidence of oxidant activity in bronchoalveolar lavage fluid.J. Clin. Invest. 71:754–758.

    Google Scholar 

  11. McGuire, W. W., R. G. Spragg, A. B. Cohen, andC. G. Cochrane. 1982. Studies on the pathogenesis of the adult respiratory distress syndrome.J. Clin. Invest. 69:543–553.

    Google Scholar 

  12. Zheutlin, L. M., E. J. Thonar, S. Gillespie, E. R. Jacobs, M. E. Hanley, R. A. Balk, andR. C. Bone. 1987. C3a DES ARG and elastase alpha-1-protease inhibitor levels in lavage fluid from patients with adult respiratory distress syndrome.J. Crit. Care 2:86–92.

    Google Scholar 

  13. Baird, B. R., J. C. Cheronis, R. A. Sandhaus, E. M. Berger, C. W. White, andJ. E. Repine. 1986. O2 metabolites and neutrophil elastase synergistically cause edematous injury in isolated rat lungs.J. Appl. Physiol. 61:2224–2229.

    Google Scholar 

  14. Rodell, T. C., J. C. Cheronis, C. L. Ohnemus, D. J. Piermattei, andJ. E. Repine. 1987. Xanthine oxidase mediates elastase-induced injury to isolated lungs and endothelium.J. Appl. Physiol. 63:2159–2163.

    Google Scholar 

  15. Bowman, C. M., E. N. Butler, andJ. E. Repine. 1983. Hyperoxia damages cultured endothelial cells causing increased neutrophil adherance.Am. Rev. Respir. Dis. 128:469–472.

    Google Scholar 

  16. Bieth, J., B. Spiess, andC. G. Wermuth. 1974. The synthesis and analytic use of a highly sensitive and convenient substrate for elastase.Bioch. Med. 11:350–357.

    Google Scholar 

  17. Carp, H., andA. Janoff. 1980. Potential mediators of inflammation. Phagocytic-derived oxidants suppress the elastase inhibitory capacity of alpha-1-protease inhibitor in vitro.J. Clin. Invest. 66:987–995.

    Google Scholar 

  18. Nagy, Z., H. Peters, andI. Huttner. 1983. Charge-related alterations of the cerebral endothelium.Lab. Invest. 49:662–671.

    Google Scholar 

  19. Carlson, J. A., B. B. Rogers, R. N. Sifers, H. K. Hawkins, M. J. Finegold, andS. L. Woo. 1988. Multiple tissues express alpha-1-antitrypsin in transgenic mice and man.J. Clin. Invest. 82:26–36.

    Google Scholar 

  20. Dziegielewska, K. M., N. R. Saunders, E. J. Schetjer, H. Zakut, D. Zevin-Jonkin, R. Zisling, andH. Sorez. 1986. Synthesis of plasma proteins in fetal, adult, and neoplastic human brain tissue.Dev. Biol. 115:93–104.

    Google Scholar 

  21. Geboes, F., M. B. Ray, P. Rutgeerts, F. Callea, V. J. Desmet, andG. Vantrappen. 1982. Morphologic identification of alpha-1-antitrypsin in human cell intestine.Histopathology (Oxf)6:55–60.

    Google Scholar 

  22. Kittas, C., K. Aroni, A. Mantani, andC. S. Papadimitriou. 1982. Immunocytochemical demonstration of alpha-1-antitrypsin and alpha-1-antichymotrypsin in human gastrointestinal tract.Hepatogastroenterology 29:275–277.

    Google Scholar 

  23. Ray, M. B., V. J. Desmet, andW. Gepts. 1977. Alpha-1-antitrypsin immunoreactivity in islet cells of human pancreas.Cell Tissue Res. 185:63–68.

    Google Scholar 

  24. Tahara, E., H. Ito, K. Taniyama, H. Yokozaki, andJ. Hata. 1984. Alpha-1-antitrypsin, alpha-antichymotrypsin, and alpha-2-macroglobulin in human gastric carcinomas: a restrospec- tive immunohistochemical study.Human Pathology 15:957–964.

    Google Scholar 

  25. Mornex, J. F., A. Chytil-Weir, Y. Martinet, M. Courtney, J. P. LeCocq, andR. G. Crystal. 1986. Expression of the alpha-1-antitrypsin gene in mononuclear phagocytes of normal and alpha-1-antitrypsin-deficient individuals.J. Clin. Invest. 77:1952–1961.

    Google Scholar 

  26. Perlmutter, D. H., L. T. May, andP. B. Sehgal. 1989. Interferon B2/interleukin 6 modulates synthesis of α1-antitrypsin in human mononuclear phagocytes and in human hepatoma cells.J. Clin. Invest. 84:138–144.

    Google Scholar 

  27. Du Bois, R. M., J.-F. Bernaudin, P. Paako, R. Hubbard, H. Takahashi, U. Ferrans, andR. G. Crystal. 1991. Human neutrophils express the α-1-antitrypsin gene and produce α-1-antitrypsin.Blood 77:2724–2730.

    Google Scholar 

  28. Rao, C. N., D. E. Gomez, D. T. Woodley, andU. P. Thorgeirsson. 1995. Partial characterization of novel serine proteinase inhibitors from human umbilical vein endothelial cells.Arch. Biochem. and Biophys. 319:55–62.

    Google Scholar 

Download references

Author information

Author notes

  1. Michael E. Hanley

    Present address: University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Box C-322, 80262, Denver, Colorado

Authors and Affiliations

  1. Webb- Waring Institute for Biomedical Research, Departments of Medicine at Denver General Hospital, University of Colorado Health Sciences Center, 80262, Denver, Colorado

    Michael E. Hanley, Lance S. Terada, John C. Cheronis & John E. Repine

Authors
  1. Michael E. Hanley
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lance S. Terada
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. John C. Cheronis
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. John E. Repine
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hanley, M.E., Terada, L.S., Cheronis, J.C. et al. Endothelial cell associated anti-elastolytic activity. Inflammation 20, 327–337 (1996). https://doi.org/10.1007/BF01488207

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01488207

Keywords

Search

Navigation