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Phagocytosis ofPseudomonas aeruginosa fails to elicit heat shock protein expression in human monocytes

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Abstract

Phagocytosis represents a powerful stress for the phagocytic cells. Phagocytosis ofStaphylococcus aureus induces a stress response associated with the synthesis of specific heat shock/stress proteins (HSP). Here we investigated the stress response of human monocyte-macrophages (mφ) toPseudomonas aeruginosa, a bacterium found, as forS. aureus, in the airways of patients suffering cystic fibrosis.P. aeruginosa activated in mφ the production of both extra- and intracellular O −2 , increased Interleukin-1β and actin, but failed to induce host HSP. NeitherS. aureus' exotoxins nor the scavenging property ofP. aeruginosa's alginate, but the lower toxicity ofP. aeruginosa and/or differential activation of proteine kinase C (PKC) by the two bacteria, might explain their differences in host HSP induction. While O −2 is insufficient to induce HSP synthesis in mφ, hydroxyl radicals, generated in the presence of exogenous iron, is a likely additional signal, along with PKC activation, for HSP induction during bacterial phagocytosis.

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Authors and Affiliations

  1. Allergy Unit, University Hospital, 1211, Geneva 14, Switzerland

    Constance Barazzone, Salomé Kantengwa & Barbara S. Polla

  2. Department of Pediatrics, University Hospital, 1211, Geneva 14, Switzerland

    Constance Barazzone & Susanne Suter

  3. Laboratoire de Physiologie respiratoire, UFR Cochin Port-Royal, 24 rue du Faubourg St. Jacques, 75014, Paris

    Barbara S. Polla

Authors
  1. Constance Barazzone
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  2. Salomé Kantengwa
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  3. Susanne Suter
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  4. Barbara S. Polla
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Barazzone, C., Kantengwa, S., Suter, S. et al. Phagocytosis ofPseudomonas aeruginosa fails to elicit heat shock protein expression in human monocytes. Inflammation 20, 243–262 (1996). https://doi.org/10.1007/BF01488202

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