Summary
The activities of 11 enzymes of energy-linked metabolism (phosphoglucomutase, hexokinase, glucose-6-phosphate dehydrogenase, glycerol-3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, lactate dehydrogenase, malic enzyme, malate dehydrogenase, glutamate dehydrogenase, succinate dehydrogenase, glycerol-3-phosphate oxidase) were determined semiquantitatively by histochemical techniques in bone marrow cells of insulin-dependent human diabetics as well as healthy controls.
In diabetics enzyme activities were found to be elevated in cells of erythropoesis. Three groups of enzymes can be separated: in the first group activities are increased only in the precursor cells of erythropoesis, in a second group enzyme activities at latter stages are elevated while a third group demonstrates elevated activities at all stages of cell proliferation. Only minor differences are observed in cells of granulopoesis and in megakaryocytes.
These data suggest that insulin deficiency or diabetic deviations in the metabolic state affect early cytogenesis in the bone marrow as well as later stages in peripheral blood cells.
Zusammenfassung
In Knochenmarkszellen von insulinbedürftigen Diabetikern werden 11 Enzyme des energieliefernden Stoffwechsels (PGluM, HK, G6PDH, GDH, GAPH, LDH, ME, MDH, GluDH, SuDH, GPOx) halbquantitativ bestimmt. In den Zellen der Erythropoese sind die Enzymaktivitäten höher als bei Kontrollen. Es lassen sich 3 Typen von Enzymen herausstellen, und zwar Enzyme, die in den ersten Vorstufen in ihrer Aktivität erhöht sind, später erst ansteigen, und solche, die immer höher sind als die Enzymaktivitäten von Kontrollen. Die Granulopoese und die Megakaryocyten zeigen nur geringe Unterschiede.
Demnach gibt es offenbar Effekte des Insulinmangels oder Auswirkung der diabetischen Stoffwechselstörung, die bereits im Knochenmark und andere, die erst in der Peripherie im Stoffwechsel der Blutzellen nachweisbar werden.
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Literatur
Mitzkat, H. J., Wiegrefe, K., Meyer, U.: Enzyme patterns of energy-linked metabolism in blood cells of human diabetics. Hormone and Metabolic Research (1972) (im Druck).
Pette, D., Brandau, H.: Enzymhistogramme und Enzymaktivitäten der Rattenleber. Enzym. biol. clin.6, 79 (1966).
Zurwehme, D., Mitzkat, H. J., Poliwoda, H., Dommes, B.: Enzyme histochemistry of the energy metabolism in the cells of normal human bone marrow. Acta haemat. (Basel)46, 24 (1971).
Barthels, H.: Enzymmuster der Erythrocyten bei normalen und mongoloiden Kindern. Fortschr. Med.88, 986 (1970).
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Mitzkat, H.J., Zurwehme, D. Halbquantitative histochemische Aktivitätsbestimmung von Enzymen des energieliefernden Stoffwechsels in Knochenmarkszellen von Diabetikern. Klin Wochenschr 50, 562–565 (1972). https://doi.org/10.1007/BF01487725
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DOI: https://doi.org/10.1007/BF01487725
Key words
- Histochemical measurement
- energy-linked metabolism
- Phosphoglucomutase (E.C. 2.7.51)
- Hexokinase (E.C. 2.7.1.1)
- Glucose-6-phosphate dehydrogenase (E.C. 1.1.1.49)
- Glycerol-3-phosphate dehydrogenase (E.C. 1.1.1.8)
- Glyceraldehyde-3-phosphate dehydrogenase (E.C. 1.2.1.12)
- Lactate dehydrogenase (E.C. 1.1.1.27)
- Malic enzyme (E.C. 1.1.1.40)
- Malate dehydrogenase (E.C. 1.1.1.37)
- Glutamate dehydrogenase (E.C. 1.4.1.2)
- Succinate dehydrogenase (E.C. 1.3.99.1)
- Glycerol-3-phosphate oxidase (E.C. 1.1.99.5)
- bone marrow cells
- human insulin-dependent diabetics
Schlüsselwörter
- Histochemische Enzymaktivitätsbestimmung
- energieliefernder Stoffwechsel
- Phosphoglucomutase (E.C. 2.7.51)
- Hexokinase (E.C. 2.7.1.1)
- Glucose-6-phosphat-Dehydrogenase (E.C. 1.1.1.49)
- Glycerin-3-phosphat-Dehydrogenase (E.C. 1.1.1.8)
- Glyceraldehyd-3-phosphat-Dehydrogenase (E.C. 1.2.1.12) Laktatdehydrogenase (E.C. 1.1.1.27)
- Malic enzyme (E.C. 1.1.1.40)
- Malatdehydrogenase (E.C. 1.1.1.37)
- Glutamatdehydrogenase (E.C. 1.4.1.2)
- Succinatdehydrogenase (E.C. 1.3.99.1)
- Glycerin-3-phosphat-Oxydase (E.C. 1.1.99.5)
- Knochenmarkzellen
- insulinbedürftige Diabetiker