Skip to main content

Advertisement

SpringerLink
Log in

Secretion of type-1-fimbriae binding proteins from human neutrophil granulocytes

  • Original Articles
  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

Granule matrix proteins secreted from human neutrophils after ionomycin stimulation were separated by SDS-PAGE, blotted onto a polyvinylidene diflouride (PVDF) membrane and overlaid with the mannose-binding lectin concanavalin A (Con A) orEscherichia coli bacteria exposing type-1-fimbriae. Four proteins of approximately 30, 40, 70 and 80 kD, respectively, derived from both the azurophil and the specific granules were shown to expose mannose-containing stuctures by binding of Con A. Such reactivity was also shown for a 90-kD protein from the light membrane fraction enriched in plasma membrane and secretory vesicles. When blots of granule matrix proteins were exposed to type-1-fimbriated bacteria, a total of seven proteins was recognized; four of the five Con A-binding proteins (40. 70, 80 and 90 kD) was detected also by the bacteria in addition to three proteins not detected by Con A (50, 60 and 100 kD). The role of the secreted type-1-fimbriae binding proteins as anti-adhesin candidates is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Henson, P. M., J. E. Henson, C. Fittchen, D. L. Bratton, andD. W. H. Riches. 1992. Degranulation and secretion by phagocytic cells.In Inflammation: basic principles and clinical correlates. 2nd ed. J. I. Gallin, I. M. Goldstein, and R. Snyderman, editors. Raven Press Ltd., New York, pp. 511–539.

    Google Scholar 

  2. Klebanoff, S. J. 1992. Oxygen metabolites from phagocytes.In Inflammation. J. I. Gallin, I. M. Goldstein, and R. Snyderman, editors. Raven Press Ltd, New York, pp. 541–588.

    Google Scholar 

  3. Greenberg, S., andS. C. Silverstein. 1993. Phagocytosis.In Fundamental Immunology. 3rd ed. W. E. Paul, editor. Raven Press Ltd, New York, pp. 941–964.

    Google Scholar 

  4. Ofek, I., andN. Sharon. 1988. Lectinophagocytosis: a molecular mechanism of recognition between cell surface sugars and lectins in the phagocytosis of bacteria.Infect. Immun. 56:539–547.

    Google Scholar 

  5. Bar-Shavit, Z., I. Ofek, R. Goldman, D. Mirelman, andN. Sharon. 1977. Mannose residues on phagocytes as receptors for the attachment ofEscherichia coli andSalmonella typhi.Biochem. Biophys. Res. Comm. 78:455–460.

    Google Scholar 

  6. Blumenstock, E., andK. Jann. 1982. Adhesion of piliatedEscherichia coli strains to phagocytes: differences between bacteria with mannose-sensitive pili and those with mannose-resistant pili.Infect. Immun. 35:264–269.

    Google Scholar 

  7. Karlsson, A., M. Markfjäll, H. Lundqvist, N. Strömberg, andC. Dahlgren. 1995. Detection of glycoprotein receptors on blotting membranes by binding of live bacteria and amplification by growth.Anal. Biochem. 224:390–394.

    Google Scholar 

  8. Öhman, L., J. Hed, andO. Stendahl. 1982. Interaction between human polymorphonuclear leukocytes and two different strains of type 1 fimbriae-bearingEscherichia coli.J. Infect. Dis. 146:751–757.

    Google Scholar 

  9. Sengeløv, H., M. H. Nielson, andN. Borregaard. 1992. Separation of human neutrophil plasma membrane from intracellular vesicles containing alkaline phosphatase and NADPH oxidase activity by free flow electrophoresis.J. Biol. Chem. 267:14912–14917.

    Google Scholar 

  10. Bainton, D. F. 1973. Sequential degranulation of the two types of polymorphonuclear leukocyte granules during phagocytosis of microorganisms.J. Cell Biol. 58:249–264.

    Google Scholar 

  11. Bentwood, B. J., andP. M. Henson. 1980. The sequential release of granule constituents from human neutrophils.J. Immunol. 124:855–862.

    Google Scholar 

  12. Steadman, R., N. Topley, D. W. Jenner, M. Davies, andJ. D. Williams. 1988. Type 1 fimbriateEscherichia coli stimulates a unique pattern of degranulation by human polymorphonuclear leukocytes.Infect. Immun. 56:815–822.

    Google Scholar 

  13. Fletcher, M. P., andJ. I. Gallin. 1983. Human neutrophils contain an intracellular pool of putative receptors for the chemoattractant N-formyl-methionyl-leucyl-phenylalanine.Blood 62:792–9.

    Google Scholar 

  14. Todd, R. III.,M. A. Arnaout, R. E. Rosin, C. A. Crowley, W. A. Peters, andB. M. Babior. 1984. Subcellular localization of the large subunit of Mol (Mol alpha; formerly gp 110), a surface glycoprotein associated with neutrophil adhesion.J. Clin. Invest. 74:1280–1290.

    Google Scholar 

  15. Miller, L. J., D. F. Bainton, N. Borregaard, andT. A. Springer. 1987. Stimulated mobilization of monocyte Mac-1 and p150,95 adhesion proteins from an intracellular vesicular compartment to the cell surface.J. Clin. Invest. 80:535–544.

    Google Scholar 

  16. Kuijpers, T. W., A. T. Tool, D. S. C. L. A. van Ginsel, J. J. Underwater, D. Roos, andA. J. Verhoeven. 1991. Membrane surface antigen expression on neutrophils: a reappraisal of the use of surface markers for neutrophil activation.Blood 78:1105–1111.

    Google Scholar 

  17. Farrell, C. F., andR. F. Rest. 1990. Up-regulation of human neutrophil receptors forNeisseria gonorrhoeae expressing PII outer membrane proteins.Infect. Immun. 58:2777–2784.

    Google Scholar 

  18. Böyum, A. 1968. Isolation of mononuclear cells and granulocytes from human blood.Scand. J. Lab. Invest. 21:77–89.

    Google Scholar 

  19. Amrein, P. C., andT. P. Stossel. 1980. Prevention of degradation of human polymorphonuclear leukocyte proteins by diisopropylfluorophosphate.Blood 56:442–447.

    Google Scholar 

  20. Borregaard, N., J. M. Heiple, E. R. Simons, andR. A. Clark. 1983. Subcellular localization of the b-cytochrome component of the human neutrophil microbicidal oxidase: translocation during activation.J. Cell Biol. 97:52–61.

    Google Scholar 

  21. Bos, A., R. Weaver, andD. Roos. 1978. Characterization and quantification of the peroxidase in human monocytes.Biochem. Biophys. Acta. 525:37–44.

    Google Scholar 

  22. DeChatelet, L. R., andM. R. Cooper. 1970. A modified procedure for the determination of leukocyte alkaline phosphatase.Biochem. Med. 4:61–68.

    Google Scholar 

  23. Gottlieb, C., K. Lau, L. R. Wassermann, andV. Herbert. 1965. Rapid charcoal assay for intrinsic factor (IF), gastric juice unsaturated B12 binding capacity, antibody to IF, and serum unsaturated B12 binding capacity.J. Hematol. 25:875–883.

    Google Scholar 

  24. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature 227:680–685.

    Google Scholar 

  25. Burnette, W. N. 1981. “Western blotting”: electrophoretic transfer of proteins from sodium dodecyl sulfate—polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.Anal. Biochem. 112:195–203.

    Google Scholar 

  26. Sauter, S. L., S. M. Rutherfurd, C. Wagener, J. E. Shively, andS. A. Hefta. 1991. Binding of nonspecific cross-reacting antigen, a granulocyte membrane glycoprotein, toEscherichia coli expressing type 1 fimbriae.Infect. Immun. 59:2485–2493.

    Google Scholar 

  27. Gbarah, A., C. G. Gahmberg, I. Ofek, U. Jacobi, andN. Sharon. 1991. Identification of the leukocyte adhesion molecules CD11 and CD18 as receptors for type 1-fimbriated (mannose-specific)Escherichia coli.Infect. Immun. 59:4524–4530.

    Google Scholar 

  28. Sauter, S. L., S. M. Rutherfurd, C. Wagener, J. E. Shively, andS. A. Hefta. 1993. Identification of the specific oligosaccharide sites recognized by type 1 fimbriae fromEscherichia coli on nonspecific cross-reacting antigen, a CD66 cluster granulocyte glycoprotein.J. Biol. Chem. 268:15510–15516.

    Google Scholar 

  29. Firon, N., I. Ofek, andN. Sharon. 1983. Carbohydrate specificity of the surface lectins ofEscherichia coli, Klebsiella pneumoniae, andSalmonella typhimurium.Carbohydr. Res. 120:235–249.

    Google Scholar 

  30. Ofek, I., andN. Sharon. 1990. Adhesins as lectins: specificity and role in infection.Curr. Top. Microbiol. Immunol. 151:91–113.

    Google Scholar 

  31. Baenziger, J. U., andD. Fiete. 1979. Structural determinants of Concanavalin A specificity for oligosaccharides.J. Biol. Chem. 254:2400–2407.

    Google Scholar 

  32. Borregaard, N., K. Lollike, L. Kjeldsen, H. Sengeløv, L. Bastholm, M. H. Nielson, andD. F. Bainton. 1993. Human neutrophil granules and secretory vesicles.Eur. J. Haematol. 51:187–198.

    Google Scholar 

  33. Ying, L., andP. Furmanski. 1993. Iron binding to human lactoferrin alters reactivity of the protein with plant lectins.Biochem. Biophys. Res. Commun. 196:686–691.

    Google Scholar 

  34. Moguilevsky, N., L. A. Retegui, andP. L. Masson. 1985. Comparison of human lactoferrins from milk and neutrophilic leucocytes. Relative molecular mass, isoelectric point, iron-binding properties and uptake by the liver.Biochem. J. 229:353–359.

    Google Scholar 

  35. Gottschalk, S., A. Waheed, andK. von Figura. 1989. Targeting of lysosomal acid phosphatase with altered carbohydrate.Biol. Chem. Hoppe Seyler 370:75–80.

    Google Scholar 

  36. Burnouf, T., J. Constans, A. Clerc, J. Descamps, L. Martinache, andM. Goudemand. 1987. Biochemical and biological properties of an alpha 1-antitrypsin concentrate.Vox Sang. 52:291–297.

    Google Scholar 

  37. Travis, J., andG. S. Salvesen. 1983. Human plasma proteinase inhibitors.Annu. Rev. Biochem. 52:655–709.

    Google Scholar 

  38. Pereira, H. A. 1995. CAP37, a neutrophil-derived multifunctional inflammatory mediator.J. Leukoc. Biol. 57:805–812.

    Google Scholar 

  39. von Clausbruch, U., andH. Tschesche. 1988. Cathepsin D from human leukocytes. Purification by affinity chromatography and properties of the enzyme.Biol. Chem. Hoppe Seyler 369:683–691.

    Google Scholar 

  40. Gulnik, S., E. T. Baldwin, N. Tarasova, andJ. Erickson. 1992. Human liver cathepsin D. Purification, crystallization and preliminary X-ray diffraction analysis of a lysosomal enzyme.J. Mol. Biol. 227:265–270.

    Google Scholar 

  41. Knauper, V., S. Kramer, H. Reinke, andH. Tschesche. 1990. Chacterization and activation of procollagenase from human polymorphonuclear leucocytes. N-terminal sequence determination of the proenzyme and various proteolytically activated forms.Eur. J. Biochem. 189:295–300.

    Google Scholar 

  42. Hasty, K. A., M. S. Hibbs, A. H. Kano, andC. L. Mainardi. 1986. Secreted forms of human neutrophil collagenase.J. Biol. Chem. 261:5645–5650.

    Google Scholar 

  43. Parkkinen, J., R. Virkola, andT. K. Korhonen. 1988. Identification of factors in human urine that inhibit the binding ofEscherichia coli adhesins.Infect. Immun. 56:2623–2630.

    Google Scholar 

  44. Babu, J. P., S. N. Abraham, M. K. Dabbous, andE. H. Beachey. 1986. Interaction of a 60-kilodalton D-mannose-containing salivary glycoprotein with type 1 fimbriae ofEscherichia coli.Infect. Immun. 54:104–108.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. The Phagocyte Research Laboratory Department of Medical Microbiology Immunology, University of Göteborg, S-413 46, Göteborg, Sweden

    Anna Karlsson & Claes Dahlgren

Authors
  1. Anna Karlsson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Claes Dahlgren
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Karlsson, A., Dahlgren, C. Secretion of type-1-fimbriae binding proteins from human neutrophil granulocytes. Inflammation 20, 389–400 (1996). https://doi.org/10.1007/BF01486741

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01486741

Keywords

Search

Navigation