Summary
Phytohaemagglutinin (PHA-P) was separated by ion exchange chromatography, absorption procedures, and isoelectric focusing. Two active fractions were obtained. One of them was homogenous by physico-chemical characterization. Both fractions showed lymphoagglutinating, mitogenic, and cytoaggression-inducing activity. In spite of varying isoelectric points, the biological properties were qualitatively identical. It was not possible to associate single properties to different fractions. In our preparation, the biological activity was related to protein and to the protein content of the fractions. From other experimental data, we think that the binding sites of the active molecule are represented by carbohydrates.
Zusammenfassung
PHA-P wurde durch Ionenaustausch-chromatographie, Absorption der Fraktionen an Schweine-erythrocyten und isoelektrische Focussierung fraktioniert. Es fanden sich 2 aktive Fraktionen. Beide Fraktionen hatton lymphagglutinierende, mitogene und Cytoaggression induzierende Aktivität. Trotz verschiedener isoelektrischer Punkte waren die biologischen Eigenschaften der beiden Fraktionen qualitativ gleich. Es war nicht möglich, einzelne der an Lymphocyten auslösbaren Reaktionen verschiedenen Fraktionen zuzuordnen. In unserer Präparation stand die biologische Aktivität quantitativ in Relation zum Proteingehalt der Fraktionen. Auch war die biologische Wirkung an Protein geknüpft. Allerdings fehlen bisher Versuche, die biologische Aktivität vom Protein zu trennen. Wir sind aufgrund anderer Versuche davon überzeugt, daß die Bindungsstellen des aktiven Moleküls mehr durch Kohlenhydrate als durch Proteine repräsentiert werden.
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Schumacher, K., Oerkermann, H., Uhlenbruck, G. et al. Isolation of the lymphoagglutinating fractions of phytohaemagglutinin (PHA) from phaseolus vulgaris and characterization of their biological properties. Klin Wochenschr 49, 286–288 (1971). https://doi.org/10.1007/BF01485435
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DOI: https://doi.org/10.1007/BF01485435