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Leukocytenisolicrungen aus kleinen Blutvolumina

Isolation of leukocytes from small blood volumes

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Summary

Isolation of leukocytes was best obtained by adding dextran and glucose to full blood. Blood volumes from 2–15 ml allow to recover sufficient leukocytes for several biochemical investigations. -70% (± 8.9%) of the leukocytes were recovered in 90 min. Resuspension in the remaining plasma dextran was performed, adding a buffered glucose solution.—The contamination corresponds to about one erythrocyte and two thrombocytes per leukocyte, which could not be lowered by any modification of the method. Electronmicroscopy showed no morphological damage of the isolated cells.

Zusammenfassung

Die beste Isolierung der Leukocyten erreichten wir mit Dextran- und Glucosezusätzen zum Vollblut mit anschließender Zentrifugation.

Die Ausgangsblutvolumina lagen zwischen 2,0–15,0 ml Vollblut, aus denen genügend Leukocyten für die verschiedensten biochemischen Untersuchungen isoliert werden konnten. In 1 1/2 Std konnten 70% (± 8,9%) der entnommenen Leukocyten wiedergewonnen und die Resuspension in Rest-Plasma-Dextran unter Zugabe gepufferter Glucose-Lösung durchgeführt werden.

Die Kontamination lag bei ca. 1 Erythrocyten und 2 Thrombocyten pro Leukocyt, die wir mit keiner Modifikation des Verfahrens noch weiter senken konnten.

Morphologische Schädigungen der isolierten Zellen ließen sich auch in elektronenmikroskopischen Aufnahmen nicht nachweisen.

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Authors and Affiliations

  1. Universitäts-Kinderklinik Marburg a. d. Lahn und Institut für physikalische Biologie und Elektronenmikroskopie, Germany

    W. Barthelmai, M. Vetrella, C. Eschenbach & E. Jehn

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  1. W. Barthelmai
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  2. M. Vetrella
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  3. C. Eschenbach
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  4. E. Jehn
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Barthelmai, W., Vetrella, M., Eschenbach, C. et al. Leukocytenisolicrungen aus kleinen Blutvolumina. Klin Wochenschr 49, 263–268 (1971). https://doi.org/10.1007/BF01485428

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