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Elektronenmikroskopischer Enzymnachweis in Suspensionen von Zellbestandteilen

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Zusammenfassung

Es wird ein Verfahren zur elektronenmikroskopischen Darstellung der Enzymorte für die Glucose-6-Phosphatase und sauren Phosphatasen in der Mikrosomenfraktion von Leberzellen der Ratte angegeben. Das elektronendichte Reaktionsprodukt ist im Bereich von äußeren Lamellen des endoplasmatischen Reticulum rhythmisch angeordnet, jedoch bestehen bezüglich der Ablagerungsart für die beiden untersuchten Enzyme gewisse Unterschiede.

Summary

A method is described for elektronmicroscopic localisation of active sites of glucose-6-phosphatase and acid phosphatases in microsomal fraction of rat liver cells. The electron-dense reaction product is rhythmically localised on the outer lamellae of the endoplasmic reticulum. The mode of localisation differs slightly between the investigated enzymes.

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Literatur

  1. Benedetti, P., Emmelot, E. L.: Structure and function of plasma membranes isolated from liver. In: Ultrastructure in biological systems, vol. 4, The membranes, p. 33–120 (ed. A. J. Dalton and F. Haguenau). New York and London: Academic Press 1968.

    Google Scholar 

  2. Berman, H. M., Gram, W., Spirts, M. A.: An improved reproducible method of preparing rat liver plasma cell membranes in buffered isotonic sucrose. Biochim. biophys. Acta (Amst.)183, 10–18 (1969).

    Google Scholar 

  3. Dallner, G., Siekevitz, P., Palade, G. E.: Biogenesis of endoplasmic reticulum membranes. II. Synthesis of constitutive microsomal enzymes in developing rat hepatocytes. J. Cell Biol.30, 97–117 (1966).

    Google Scholar 

  4. —— Ernster, L.: Subfactionation and composition of microsomal membranes: A Review. J. Histochem. Cytochem.16, 611–632 (1968).

    Google Scholar 

  5. De Duve, C.: The lysosome concept. In: Lysosome, p. 1, (A. V. S. de Reuck and M. P. Mameron). London: J. A. Churchill 1963.

    Google Scholar 

  6. Diamantstein, T.: Persönliche Mitteilung 1969.

  7. Ericsson, J. L. E., Jakobsson, S.: Fine structural lokalization of glucose-6-phosphatase in renal proximal tubules, p. 53–54. 3rd Int. Congr. Histochem. Cytochem., New York, Aug. 18–22, 1968.

  8. Fishman, W. H., Goldman, S. S., Lellis, R. De: Dual localization of β glucuronidase in endoplasmic reticulum and in lysosomes. Nature (Lond.),213, 457–460 (1967).

    Google Scholar 

  9. Fiske, C. H., Subbarow, Y. Zit. in Handbuch der physiologischen und pathologisch-chemischen Analyse (Hrsg. Hoppe-Seyler, Thierfelder), Bd. 3, S. 465. Berlin-Göttingen-Heidelberg: Springer, 1955.

    Google Scholar 

  10. Horne, R. W., Brenner, S.: A negative staining technique for high resolution of virus. In: IV. Int. Congr. E. M. Berlin 1958, Bd. II, S. 625.

  11. Huxley, H. E., Zubay, G.: Electron microscopic observations on the structure of microsomal particles from E. coli. J. molec. Biol.2, 10 (1960).

    Google Scholar 

  12. Lowry, O. H., Rosebrough, N. J., Farr, L., Randall, R. J.: Protein measurement with the Folin reagent.: J. biol. Chem.193, 265 (1951).

    Google Scholar 

  13. Orrenius, S. S., Ericsson, J. L. E.: On the relationship of liver glucose-6-phosphatase to the proliferation of endoplasmic reticulum in phenobarbital induction. J. Cell Biol.31, 243–256 (1966).

    Google Scholar 

  14. Robertson, J. D.: The unit membrane. In: Electron microscopy in anatomy p. 74–99 (J. D. Boyd, F. R. Johnson and J. D. Lever). London: Edward Arnold 1961.

    Google Scholar 

  15. Sjoestrand, F. S.: Ultrastructure and function of cellular membranes. In: Ultrastructure in biological systems, vol. 4, The membranes, p. 151–210, ed. by A. J. Dalton and F. Haguenau. New York and London: Acaemic Press 1968.

    Google Scholar 

  16. Swanson, M. A. Zit. in: Handbuch der physiologischen und pathologisch-chemischen Analyse. (Hrsg. Hoppe-Seyler-Thierfelder), Bd. 6/B, S. 994. Berlin-Heidelberg-New York: Springer, 1966.

    Google Scholar 

  17. Swift, H.: The fine structure of annulate lamellae. J. biophys. biochem. Cytol.2, Suppl. 415–418 (1956).

    Google Scholar 

  18. Valentine, R. C., Horne, R. W.: An assessment of negative staining techniques for revealing ultrastructure In: Symposia of the int. society for cell biology, vol. 1, p. 263–278, The interpretation of ultrastructure, ed. by R. J. C. Harris. New York and London: Academic Press 1962.

    Google Scholar 

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Authors and Affiliations

  1. Institut für Pathologie des Klinikum Steglitz der Freien Universität Berlin, Germany

    H. -J. Stolpmann & U. M. Gross

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  1. H. -J. Stolpmann
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  2. U. M. Gross
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Stolpmann, H.J., Gross, U.M. Elektronenmikroskopischer Enzymnachweis in Suspensionen von Zellbestandteilen. Klin Wochenschr 48, 209–212 (1970). https://doi.org/10.1007/BF01485058

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  • DOI: https://doi.org/10.1007/BF01485058

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