Zusammenfassung
Die Wirkung von E. coli-L-Asparaginase auf die Nucleinsäure- und Proteinbiosynthese wurde an Zellsuspensionen lymphatischer Leukämiezellen und verschiedener Transplantationstumoren untersucht. Hierbei wurde beobachtet, daß dieses Enzym eine deutliche Senkung des14C-Thymidineinbaus an Leukämiezellen des Rindes hervorruft, ein Effekt, welcher durch Zusatz von L-Asparagin und L-Glutamin nicht aufhebbar war. Bei Zellen des Walker- und Yoshida-Ascitestumors der Ratte fanden sich eine Hemmung der14C-Thymidin- und14C-Leucininkorporation, während die L-Asparaginase bei Asciteszellen des Crocker-Sarkoms der Maus lediglich eine Senkung des14C-Leucineinbaus bewirkte. Die Ursache für das Verhalten des14C-Thymidineinbaus bei bestimmten Tumorzellen bleibt zunächst ungeklärt.
Summary
Ascites cells derived from various transplantation tumours and leukemic lymphocytes have been employed to study the in-vitro effects of E. coli L-asparaginase on protein and nucleic acid biosynthesis. Bovine lymphocytes obtained from a cow suffering from chronic lymphatic leukemia showed a marked inhibition of14C-thymidine incorporation not reversible by L-asparagine and L-glutamine addition. With Walker ascites and Yoshida ascites of the rat, this effect has been found to be accompanied by a comparable inhibition of14C-leucine uptake, while in cells from mouse crocker ascites under the same conditions only14C-leucine incorporation was decreased. Although some specific investigations have been performed on nucleic acid biosynthesis in presence of L-asparaginase, the mechanisms of action — with respect to the rapid inhibition of14C-thymidine uptake into lymphatic cells — are still unknown.
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Hirschmann, W.D., Seeber, S. & Warnecke, P. In vitro-Untersuchungen mit E. coli-L-Asparaginase an lymphatischen Rinderleukämiezellen und verschiedenen Transplantationstumoren. Klin Wochenschr 47, 1217–1220 (1969). https://doi.org/10.1007/BF01484887
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DOI: https://doi.org/10.1007/BF01484887