Zusammenfassung
Cytogenetische Untersuchungen sind bei Patienten mit Knochenmarkinsuffizienz häufig nicht möglich, da nur wenige oder keine Zellen in Mitose vorhanden sind. Mit Hilfe von Suspensions-Kulturen hämopoetischer Zellen konnte jedoch selbst in denjenigen Fällen eine große Zahl von Mitosen gewonnen werden, bei denen mit der direkten Präparationstechnik keine Mitosen gefunden werden. Durch cytogenetische Untersuchungen konnte nachgewiesen werden, daß die gewonnenen Mitosen aneuploid und somit leukämischen Ursprungs sind. Die Kombination von Kurzzeit-Suspensions-Kulturen und Chromosomenanalyse ist in bestimmten Fällen mit Knochenmarkinsuffzienz von diagnostischer Bedeutung.
Summary
In patients with haemopoietic failure, the low incidence of cells in mitosis often prevents successful chromosome preparations. By performing liquid cultures of myeloid cells a high incidence of mitoses could be obtained even in those cases failing to provide mitoses when the direct preparation technique was used. The cytogenetic studies demonstrated that the mitoses achieved by culture techniques were aneuploid and, thus, there is evidence that they are of leukaemic origin. The combination of short-term liquid culture and chromosome analysis appears to be a diagnostic approach in selected cases of hemopoietic insufficiency.
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Supported by Deutsche Forschungsgemeinschaft, SFB 1121 and SFB 682
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Heit, W., Hellriegel, K.P., Heimpel, H. et al. Improvement of preparative conditions for cytogenetic studies by short-term liquid culture of haemopoietic cells. Klin Wochenschr 55, 895–898 (1977). https://doi.org/10.1007/BF01478824
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DOI: https://doi.org/10.1007/BF01478824
Schlüsselwörter
- Cytogenetische Untersuchungen
- Suspensions-Kulturen, Agar-Kulturen
- Koloniebildende Zellen, Kolonie-stimulierende Aktivität
- Leukämie, myeloproliferative Erkrankungen, Knochenmarkinsuffizienz