Summary
Blood samples were obtained from 16 patients with primary or secondary hyperparathyroidism by selectively catheterising cervical and thyroid veins. Immunoreactive parathyroid hormone was measured with two aminoterminal (anti-N)- and three carboxyl-terminal (anti-C)-specific antisera. Two anti-C sera and one anti-N serum were useful in localising the parathyroid tumors. In addition, the parathyroid hormone fragments were separated by gelfiltration and the immunoreactivity in the effluent was estimated with anti-N and anti-C sera. The results suggest that the usefulness of antisera for parathyroid tissue localisation is determined by their affinity for the intact hormone, regardless of their anti-N or anti-C qualities.
Zusammenfassung
Bei 16 Patienten mit primärem oder sekundärem Hyperparathyreoidismus wurden selektiv Blutproben aus den Hals- und Schilddrüsenvenen gewonnen. Das immunreaktive Parathormon wurde mit zwei aminoterminal-(Anti-N)- und drei caboxylterminal-(Anti-C)-spezifischen Antiseren gemessen. Zwei Anti-C Seren und ein Anti-N-Serum erwiesen sich als geeignet zur Lokalisation der Nebenschilddrüsentumoren. Zusätzlich wurden die Parathormonfragmente durch Gelfiltration aufgetrennt, die Immunreaktivität in den Eluaten wurde mit Anti-N-und Anti-C-Seren bestimmt. Die Ergebnisse ließen erkennen, daß die Eignung der Antiseren zur Lokalisationsuntersuchung durch ihre Affinität für intaktes Hormon bestimmt wird, unabhängig von ihrer Anti-N-oder Anti-C-Eigenschaft.
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Offermann, G., Sörensen, R. & Häring, R. Immunreaktives Parathormon im Schilddrüsenvenenblut. Klin Wochenschr 56, 1007–1012 (1978). https://doi.org/10.1007/BF01476665
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DOI: https://doi.org/10.1007/BF01476665