Summary
The isolation of viable lymphocytes from fresh human appendices is described. Under standardized experimental conditions of cell preparation no selective enrichment of either T- or B-lymphocytes was observed. The mean percentage of T-lymphocytes as judged by spontaneous rosette formation found in 34 appendices examined was 50.0%. After overnight incubation surface-Ig was detected by immunoradioautography on 46.8% of the recovered lymphocytes from 10 appendices. For the detection of surface-Ig specifically purified antibodies to heavy chains µ, γ and α were used. Of the 46.8% surface-Ig positive lymphocytes the class distribution was as follows: IgM 17.7%, IgG 24.4% and IgA 4.7%. The isolated lymphocytes showed a variable mitotic response to PHA (stimulation index ranging from 3.3 to 200.9) as well as to allogeneic lymphocytes in a one-way mixed lymphocyte culture. So far, a distinct correlation could not be found between the histological degree of inflammation and the class or mitotic response of appendix lymphocytes.
Zusammenfassung
Aus der Submukosa von frisch opierten humanen Appendices wurden die Lymphocyten isoliert und die Verteilung der T- und B-Lymphocyten untersucht. Der Anteil der T-Lymphocyten aus 34 Appendices, bestimmt durch Bildung von spontanen E-Rosetten, betrug 50,0%. Durchschnittlich ließen sich 46,8% der Lymphocyten aus 10 Appendices mit dem immunautoradiographischen Nachweis von Oberflächenimmunglobulin als B-Lymphocyten charakterisieren. Von den B-Lymphocyten waren 17,7% IgM-positiv, 24,4% IgG-positiv und 4,7% IgA-positiv. Die Lymphocyten ließen sich mit Phytohaemagglutinin (PHA) stimulieren und zeigten eine positive Reaktion in der Lymphocytenmischkultur. Die Befunde zeigen, daß Suspensionen von Appendixlymphocyten einen hohen Anteil an T-Lymphocyten besitzen, der auch funktionell als solcher zu charakterisieren ist. Eine deutliche Korrelation zwischen dem histologischen Entzündungsgrad der Appendices und der Verteilung sowie der Reaktivität ihrer Lymphocyten konnte nicht gefunden werden.
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Breucha, G., Riethmüller, G. & Rieber, E.P. Isolierung und immunologische Charakterisierung von Lymphocyten aus humanen Appendices. Klin Wochenschr 53, 1155–1159 (1975). https://doi.org/10.1007/BF01476455
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DOI: https://doi.org/10.1007/BF01476455
Key words
- Lymphocytes
- membrane-bound immunoglobulin
- phytohaemagglutinin
- mixed-lymphocyte reaction
- appendix
- appendicitis