Summary
Rabbit and thrombasthenic platelet membranes were examined by SDS polyacrylamide gel electrophoresis. Both rabbit and thrombasthenic platelets failed to spread on siliconized glass surfaces and revealed platelet membrane glycoprotein patterns quite different from those of normal human platelet membranes. Typical for normal platelet membranes are four high molecular weight glycoprotein bands. The platelet membranes from rabbits and from one thrombasthenic patient showed only the first major glycopolypeptide with an apparent molecular weight of 135 000 and 120 000 D respectively.
Other platelet membrane glycopeptides (both the carbohydrate and polypeptide moiety) were completely absent in thrombasthenia. The rabbit platelet membrane yet contains two strong polypeptides in this high molecular weight region, however, without corresponding carbohydrate moieties. Therefore, we support the view that the carbohydrate chains from two high molecular weight glycoproteins are of importance for platelet spreading on glass surfaces.
Zusammenfassung
Die Membranen von Kaninchenthrombocyten und thrombasthenischen Plättchen wurden in der Polyacrylamid-SDS-Gelelektrophorese analysiert. Beide Plättchenarten breiten sich nicht an silikonisierten Glasoberflächen aus und lassen ein Glykoproteinmuster der Membran erkennen, das deutlich von dem normaler Thrombocyten abweicht. Die normale Plättchenmembran zeigt vier hochmolekulare Glykoproteinbanden im Polyacrylamidgel. Die Membranen von Kaninchenthrombocyten und thrombasthenischen Plättchen weisen nur das erste große Glykoprotein mit einem Molekulargewicht von ca. 135 000 bzw. 120 000 D auf.
Die übrigen Membranglykopolypeptide (sowohl Kohlenhydrat- als auch Proteinanteil) fehlen bei Thrombasthenie vollständig. Die Thrombocytenmembran von Kaninchen enthält in diesem hochmolekularen Bereich zwei große Polypeptide, — vergleichbar den Humanthrombocyten, — jedoch ohne entsprechende Kohlenhydratketten. So kann angenommen werden, daß der Kohlenhydratanteil dieser Glykoproteine für die Plättchenausbreitung maßgeblich ist.
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Podolsak, B., Brunswig, D. Veränderungen der hochmolekularen Membranglykoproteine bei Thrombasthenie als Ursache der fehlenden Plättchenausbreitung?. Klin Wochenschr 54, 613–617 (1976). https://doi.org/10.1007/BF01469026
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DOI: https://doi.org/10.1007/BF01469026