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Beschreibung eines vereinfachten Makrophagenmigrationshemm-Testes zur Messung zellvermittelter Immunreaktionen beim Menschen

A simplified macrophage migration inhibition test to measure t-cell derived immunity in man

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Summary

A macrophage migration inhibition test using heterologous cells (DMMTH) is performed in an agarose-droplet technique. A suspension consisting of peritoneal exsudate cells from Guinea pig and human lymphocytes, mixed with 0,2 percent agarose is dispensed in droplets with a dispenser into culture chambers used for the leukocyte migration inhibition test. Macrophages migrate out of the gelified droplets. Migration areas can be measured by planimetry. Migration areas are exact to 8.6 percent. A significant migration inhibition, caused by migration inhibitory factors is observed after the reaction of the specific antigen with sensitized lymphocytes in the agarose droplet. The sensitivity of the test has been demonstrated in Mantoux positive persons.

Zusammenfassung

Ein direkter Makrophagenmigrationshemm-Test mit heterologen Zellen (DMMTH) wird in einer Agarose-Tropfentechnik ausgeführt. Eine Suspension aus Peritonealmakrophagen des Meerschweinchens und Humanlymphozyten wird in 0,2%iger Agarose aufgeschwemmt, die mit einer automatischen Dosiervorrichtung tropfenförmig auf Platten für den Leukozytenwanderungstest aufgetragen wird. Die aus den erstarrten Tropfen erfolgende Auswanderung der Makrophagen kann planimetrisch gemessen werden. Die Streubreite der Zellkulturen beträgt im Mittel 8,6%. Eine signifikante Wanderungshemmung durch die Freisetzung des Migrations-Inhibitions-Faktors wird beobachtet, wenn sensibilisierte Humanlymphozyten mit dem in die Agaroselösung eingebrachten spezifischen Antigen reagieren. An Mantoux-positiven Probanden wurde die Sensitivität des Testes gezeigt.

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  1. Medizinische Poliklinik der Universität Heidelberg, Germany

    K. Rehn, H. Baake & W. Hunstein

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  1. K. Rehn
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  2. H. Baake
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  3. W. Hunstein
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Rehn, K., Baake, H. & Hunstein, W. Beschreibung eines vereinfachten Makrophagenmigrationshemm-Testes zur Messung zellvermittelter Immunreaktionen beim Menschen. Klin Wochenschr 53, 397–399 (1975). https://doi.org/10.1007/BF01468680

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  • DOI: https://doi.org/10.1007/BF01468680

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