Summary
Hydrocortisone treatment as well as wholebody irradiation cause marked cellular depletion in thymus and spleen from C57 Bl mice. The resistant cell populations were found to respond better to PHA, PWM and Con A than spleen- and thymus-cell populations of untreated mice. Great differences in reactivity in the various cell populations to the three mitogens were observed. The results support the hypothesis that the immunological competent cells are among the cortisone-resistant as well as among the radioresistant cell populations.
Zusammenfassung
Hydrocortisonbehandlung und Ganzkörperbestrahlung führen bei C57 Bl-Mäusen zu einer starken Reduktion der Zellzahlen in Thymus und Milz. Die resistenten Zellpopulationen sind durch PHA, PWM und Con A deutlich besser stimulierbar als Milz- und Thymuszellen unbehandelter Mäuse, wobei die mitogene Wirkung der getesteten Substanzen sehr unterschiedlich ist. Die Ergebnisse stützen die Annahme, daß cortison- und strahlenresistente Zellpopulationen die immunkompetenten Zellen enthalten.
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Literatur
Blomgren, H., Révész, L.: Cellular composition of mouse thymus after X-ray exposure. Exp. Cell Res.51, 92 (1968)
Blomgren, H., Andersson, B.: Evidence for a small pool of immunocompetent cells in the mouse thymus. Exp. Cell Res.57, 185 (1969)
Blomgren, H., Svedmyr, E.: In vitro stimulation of mouse thymus cells by PHA and allogeneic cells. Cell. Immunol.2, 285 (1971)
Cohen, J.J., Fischbach, M., Claman, H.N.: Hydrocortisone resistance of graft vs. host activity in mouse thymus, spleen and bone marrow. J. Immunol,105, 1146 (1970)
Cohen, J.J., Claman, H.N.: Thymus-marrow immunocompetence. V. Hydrocortisone-resistant cells and processes in the hemolytic antibody response of mice. J. exp. Med.133, 1026 (1971)
Jacobson, H., Blomgren, H.: Studies on the recirculating cells in the mouse thymus. Cell. Immunol.5, 107 (1972)
Janossy, G., Greaves, M.F.: Lymphocyte activation. I. Response of T and B lymphocytes to phytomitogens. Clin. exp. Immunol.9, 483 (1971)
Ling, N.R.: Lymphocyte stimulation. Amsterdam: North Holland 1968
Moorhead, J.W., Claman, H.N.: Thymus-derived lymphocytes and hydocortisone: identification of subsets of thetabearing cells and redistribution to bone marrow. Cell. Immunol.5, 74 (1972)
Rühl, H., Kirchner, H., Bochert, G.: Kinetics of the Zn2+-stimulation of human peripheral lymphocytes. Proc. Soc. exp. Biol. (N.Y.)137, 1089 (1971)
Stobo, J.D., Rosenthal, A.S., Paul, W.E.: Functional heterogeneity of murine lymphoid cells. I. Responsiveness to and surface binding of concanavalin A and phytohemagglutinin. J. Immunol.108, 1 (1972)
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Rühl, H., Vogt, W., Rühl, U. et al. Stimulation cortison- und strahlenresistenter Mäuselymphocyten in vitro durch Phythämagglutinin-P (PHA), Pokeweed Mitogen (PWM) und Concanavalin (Con A). Klin Wochenschr 51, 1026–1027 (1973). https://doi.org/10.1007/BF01468297
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DOI: https://doi.org/10.1007/BF01468297