Skip to main content
SpringerLink
Log in

Preparative electrophoretic separation of normal and neoplastic human bone marrow cells

Trennung normaler und neoplastischer Knochenmarkzellen des Menschen mittels präparativer Elektrophorese

  • Originalien
  • Published:
Klinische Wochenschrift Aims and scope Submit manuscript

Summary

Bone marrow cells of patients with chronic myeloid leukemia, monocytic leukemia, myeloblast leukemia, chronic lymphatic leukemia, typical and atypical plasma cell myeloma and M. Waldenström were separated by free flow electrophoresis and their distribution profiles compared with profiles of normal bone marrow cells. Except for myeloma cells, the pathologic cells possess the same electrophoretic mobility as their normal counterparts. Atypical or typical myeloma cells show a much faster mobility than normal plasma cells. The importance of these results is discussed.

Zusammenfassung

Knochenmarkzellen von Patienten mit chronisch myeloischer Leukämie, Monocytenleukämie, Myeloblastenleukämie, chronisch lymphatischer Leukämie, typischem und atypischem Myelom und M. Waldenström wurden mit Hilfe der präparativen trägerfreien Ablenkungselektrophorese getrennt und ihre Trennprofile mit denen normaler Knochenmarkzellen verglichen. Mit Ausnahme der Myelomzellen wanderten die pathologischen Zellen in denselben elektrophoretischen Beweglichkeitsbereichen wie die entsprechenden normalen Zellen. Myelomzellen zeigten dagegen eine starke Erhöhung ihrer elektrophoretischen Beweglichkeit im Vergleich zu normalen Plasmazellen. Die Bedeutung dieser Befunde wird diskutiert.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

EPM:

electrophoretic mobility.

References

  1. Lehninger, A. L.: Neuronal membrane. Proc. nat. Acad. Sci. (Wash.)60, 1069 (1968).

    PubMed  Google Scholar 

  2. Revel, J. P., Ito, S.: In: The specificity of cell surfaces, eds. Davis, B. D. and L. Warren, p. 213. Englewood Cliffs, New Yersey: Prentice-Hall, Inc. 1967.

    Google Scholar 

  3. Dod, B. J., Gray, G. M.: The lipid Composition of ratliver plasma membranes. Biochim. biophys. Acta (Amst.)150, 397–404 (1968).

    PubMed  Google Scholar 

  4. Benedetti, E. L., Emmelot, P.: Studies on plasma membranes. IV. The ultrastructural localization and content of sialic acid in plasma membranes isolated from rat liver and hepatoma. J. Cell Sci.2, 499–512 (1967).

    PubMed  Google Scholar 

  5. Tillack, T. W., Scott, R. E., Marchesi, V. T.: Cell-membrane ultrastructure studied by freeze etching. Fed. Proc.20, 489 (1970).

    Google Scholar 

  6. Winzler, R. J., Harris, E. D., Pekas, D. J., Johnson, C. A., Weber, P.: Studies on glycopeptides released by trypsin from intact human erythrocytes. Biochemistry (Wash.)6, 2195–2202 (1967).

    PubMed  Google Scholar 

  7. Reviewed by Mehrishi, J. N.: Molecular aspects of the mammalian cell surface. In: Progress in Biophysic and Molecular Biology, ed. Butler, J. A. V., Noble, D., p. 1–70. Oxford: Pergamon Press 1972.

    Google Scholar 

  8. Muramatsu, T., Nathenson, S. G.: Isolation of a chromatographically unique glycopeptide from murine histocompatibility -2 (H-2) membrane alloantigens labelled with H3-fucose of H3-glucosamine. Biochem. biophys. Res. Commun.38, 1–8 (1970).

    PubMed  Google Scholar 

  9. Sanford, B. H.: An alteration in tumor histocompatibility induced by neuramidases. Transplantation5, 1273 (1967).

    PubMed  Google Scholar 

  10. Woodruff, J. J., Gesner, B. M.: The effect of neuramidase on the fate of transfused lymphocytes. J. exp. Med.129, 551–567 (1969).

    PubMed  Google Scholar 

  11. Gasic, G., Gasic, T.: Removal of sialic acid from the cell coat in tumor cells and vascular endothelium, and its effects on metastasis. Proc. nat. Acad. Sci. (Wash.)48, 1172–1177 (1962).

    PubMed  Google Scholar 

  12. Ambrose, E. J., James, A. M., Lowick, J. H. B.: Differences between electrical change carried by normal and homologous tumour cells. Nature (Lond.)177, 576–577 (1956).

    PubMed  Google Scholar 

  13. Abercrombie, M., Ambrose, E. J.: The surface properties of cancer cells: A review. Cancer Res.22, 525–548 (1962).

    PubMed  Google Scholar 

  14. Forrester, J. A., Ambrose, E. J., McPherson, A.: Electrophoretic investigations of a clone hamster fibroblasts and polyoma-transformed cells from the same population. Nature (Lond.)196, 1068–1070 (1962).

    PubMed  Google Scholar 

  15. Ruhenstroth-Bauer, G., Straub, E., Sachtleben, P., Fuhrmann, G. F.: Die elektrophoretische Beweglichkeit von Blutzellen beim Gesunden und bei Kranken. Münch. med. Wschr.103, 794–797 (1961).

    Google Scholar 

  16. Ruhenstroth-Bauer, G.: In: Cell electrophoresis, ed. by J. Ambrose, p. 66. Boston: Little-Brown 1965.

    Google Scholar 

  17. Simon-Reuss, I., Cook, G. M. W., Seaman, G. V. F., Heard, D. H.: Electrophoretic studies on some types of mammalian tissue cells. Cancer Res.24, 2038–2043 (1964).

    PubMed  Google Scholar 

  18. Vasar, P. S.: The electric charge density of human tumor cell surfaces. Lab. Invest.12, 1072–1077 (1963).

    PubMed  Google Scholar 

  19. Thomson, A. E. R., Mehrishi, J. N.: Surface properties of normal human circulating small lymphocytes and lymphocytes in chronic lymphocytic leukemia. Separation adhesiveness and electrokinetic properties. Europ. J. Cancer5, 195–206 (1969).

    Google Scholar 

  20. Robineaux, R., Bazin, S.: Etude de la mobilité électrophorétique des leucocytes humains. Sang22, 241–247 (1951).

    PubMed  Google Scholar 

  21. Lichtman, M. A., Weed, R. J.: Electrophoretic mobility and N-acetyl neuraminic acid content of human normal and leukemic lymphocytes and granulocytes. Blood35, 12–22 (1970).

    PubMed  Google Scholar 

  22. Hannig, K., Zeiller, K.: Zur Auftrennung und Charakterisierung immunkompetenter Zellen mit Hilfe der trägerfreien Ablenkungselektrophorese. Hoppe-Seylers Z. physiol. Chem.350, 467 (1969).

    PubMed  Google Scholar 

  23. Zeiller, K. Hannnig, K.: Free flow electrophoretic separation of lymphocytes: Evidence for specific organ distributions of lymphoid cells. Hoppe-Seylers Z. physiol. Chem.352, 1162 (1971).

    PubMed  Google Scholar 

  24. Zeiller, K., Hannig, K., Pascher, G.: Free flow electrophoretic separation of lymphocytes: Separation of graft versus host reactive lymphocytes of rat spleens. Hoppe-Seylers Z. physiol. Chem.352, 1168 (1971).

    PubMed  Google Scholar 

  25. Zeiller, K., Holzberg, E., Pascher, G., Hannig, K.: Free flow electrophoretic separation of T and B lymphocytes. Evidence for various subpopulations of B cells. Hoppe-Seylers Z. physiol. Chem.353, 105–110 (1972).

    PubMed  Google Scholar 

  26. Wioland, M.: Personal communication.

  27. Johannson, B., Klein, E.: Cell surface localized IgM-kappa immunoglobulin reactivity in a case of chronic lymphocytic leukemia. Clin. exp. Immunol.6, 421–428 (1970).

    PubMed  Google Scholar 

  28. Papamichail, M., Brown, J. C., Holborow, E. J.: Immunoglobulins on the surface of human lymphocytes. Lancet1971 II, 850–852.

    Google Scholar 

  29. Wilson, J. D., Nossal, G. J. V.: Identification fo human T and B lymphocytes in normal peripheral blood and in chronic lymphocytic leukemia. Lancet1971 II, 788–791.

    Google Scholar 

  30. Smith, J. L., Cowling, D. C., Barker, C. R.: Response of lymphocytes in chronic lymphocytic leukemia ot plant mitogens. Lancet1972 I, 229–233.

    Google Scholar 

  31. Quaglino, D., Cowling, D. C.: Cytochemical studies on cells from chronic lymphocytic leukemia and lymphosarcoma cultured with Phytohaemagglutinin. Brit. J. Haemat.10, 358–364 (1964).

    PubMed  Google Scholar 

  32. Janossy, G., Greaves, M. F.: Lymphocyte activation. I. Response of T and B lymphocytes to Phytomitogens. Clin. exp. Immunol.9, 483–498 (1971).

    PubMed  Google Scholar 

  33. Stockman, D. G., Gallagher, M. T., Heim, L. R., South, M. A., Trentin, J. J.: Differential stimulation of mouse lymphoid cells by Phytohaemagglutinin and Pokeweedmitogen. Proc. Soc. exp. Biol. (N. Y.)163, 980–982 (1971).

    Google Scholar 

  34. Roitt, J. M., Greaves, M. F., Torrigiani, G., Brostoff, J., Playfair, J. M. L.: The cellular basis of immunological responses. A synthesis of some current view. Lancet1969 II, 367–371.

    Google Scholar 

  35. Holzberg, E., Zeiller, K.: Electrophoretic separation of antiperoxydase synthezising cells. Abstracts, First Meeting Europ. Div. Inter. Soc. Haematol., p. 293 (1971).

Download references

Author information

Authors and Affiliations

  1. Zentrum der Inneren Medizin, Abteilung für Hämatologie, der Universität Frankfurt a. M., Germany

    J. C. F. Schubert & F. Walther

  2. Max-Planck-Institut für Eiweiß- und Lederforschung, München

    E. Holzberg, G. Pascher & K. Zeiller

Authors
  1. J. C. F. Schubert
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. Walther
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. Holzberg
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. Pascher
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. K. Zeiller
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schubert, J.C.F., Walther, F., Holzberg, E. et al. Preparative electrophoretic separation of normal and neoplastic human bone marrow cells. Klin Wochenschr 51, 327–332 (1973). https://doi.org/10.1007/BF01468098

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01468098

Key words

Schlüsselwörter

Search

Navigation