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A model mosaic membrane: Association of phospholipids and cytochrome oxidase

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Abstract

The structure and physical properties of model membranes formed from lipids and cytochromec oxidase have been examined. The lipid-depleted protein is in the form of 90 Å rods or globules. When phospholipid is added the rods swell and then. form sheets and concentric membrane vesicles. The protein is saturated with lipid at 65 g/atoms of phosphorus per mole of hemea. Electron microscope examination by negative staining, sectioning, and freeze etching indicates a 50 Å thick unit membrane with 50–60 Å protein globules in the lipid bilayer. Infrared, circular dichroism and fluorescence binding studies are consistent with globular protein units surrounded with lipid. Diolein will substitute for phospholipid but the membrane formed remains as sheets rather than vesicles. Saturated phospholipids will not interact with the oxidase to form membrane. The capacity to form membrane is specific to protein associated with the hemea, and other insoluble protein in the original oxidase preparation cannot form membrane.

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Authors and Affiliations

  1. Department of Biological Sciences, Purdue University, 47907, Lafayette, Indiana

    T. F. Chuang, Y. C. Awasthi & F. L. Crane

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  1. T. F. Chuang
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  2. Y. C. Awasthi
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  3. F. L. Crane
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Chuang, T.F., Awasthi, Y.C. & Crane, F.L. A model mosaic membrane: Association of phospholipids and cytochrome oxidase. J Bioenerg Biomembr 5, 27–72 (1973). https://doi.org/10.1007/BF01458355

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  • DOI: https://doi.org/10.1007/BF01458355

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