Abstract
B 512 F dextran fractions of different polydispersities were prepared by fractionnal precipitation and preparative elution chromatography. By combination of low angle laser light scattering (LALLS) and gel permeation chromatography (GPC), absolute average molecular weights (MWs) and molecular weight distribution functions (MWDs) were determined. Static and thermodynamical properties in terms of polymer dimensions and second virial coefficient of dilute solutions of dextran in water have been investigated. The results indicate that dextran macromolecules in water are rather compact and impenetrable coils. Measuring the disaggregation shear stress of dextran-induced red blood cell aggregates by laser light reflectometry, the macromolecular bridging energy was shown to depend upon dextran sample polydispersity. This reflects the weak and reversible character of red blood cell aggregation by dextran chains in physiological saline solution.
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Snabre P, Mills P, Bitlol M, pubmitted for publication in Micror Res
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Snabre, P., Grossmann, G.H. & Mills, P. Effects of dextran polydispersity on red blood cell aggregation. Colloid & Polymer Sci 263, 478–483 (1985). https://doi.org/10.1007/BF01458338
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DOI: https://doi.org/10.1007/BF01458338