Abstract
The variable regions of murine monoclonal anti-HBx immunoglobulin and the constant region of human antibody were cloned by reverse transcriptpolymerase chain reaction (RT-PCR). The heavy-chain and light-chain variable regions were connected and coexpressed with human constant region C-r3 and C-k3 in the reconstructed vector ofE. coli. The products showed high specificity and binding ability with HBx, which is closely associated with hepatocarcinogenesis. This makes it possible to humanize the mouse monoclonal antibodies and express the fusion protein inE. coli for potential radioimmunotherapy in patients with hepatocellular carcinoma.
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Zhou, G., Liu, KD., Tang, ZY. et al. Reconstruction and expression of chimeric anti-HBx antibody inEscherichia coli . J Cancer Res Clin Oncol 123, 325–330 (1997). https://doi.org/10.1007/BF01438308
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DOI: https://doi.org/10.1007/BF01438308