Abstract
This paper summarizes three experiments on the genetic manipulation of fitness components involved in the evolution of lifespan through the introduction of an additional copy of the gene for elongation factor EF-1α into the genome ofDrosophila melanogaster. The first experiment checked a prior claim that enhanced expression of elongation factor increased the lifespan of virgin male fruitfies. It used inbred stocks; three treatment and three control lines were available. The second experiment put one treatment and one control insert into different positions on the third chromosome, then measured the influence of six genetic backgrounds on treatment effects in healthier flies. The third experiment put six treatment and six control inserts into the genetic background whose lifespan was most sensitive to the effects of treatment in the second experiment, then measured the influence of insert positions on treatment effects in healthy flies. The treatment never increased the lifespan of virgin males. It increased the lifespan of mated females in inbred flies reared to eclosion at 25°, reduced it in the positions experiment, and made no difference to lifespan in the backgrounds experiment. When it increased lifespan, it reduced fecundity. In inbred flies and in the positions experiment, the treatment reduced dry weight at eclosion of females. Marginal effects of gene substitutions on tradeoffs were measured directly. The results suggest that enhanced expression of elongation factor makes local changes within the bounds of tradeoffs that are given by a pre-existing physiological structure whose basic nature is not changed by the treatment.
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Bellen, H. J., C. J. O'Kane, C. Wilson, U. Grossniklaus, R. K. Pearson & W. J. Gehring, 1989. P-element-mediated enhancer detection: a versatile method to study development inDrosophila. Genes and Development 3: 1288–1300.
Charlesworth, B. & J. A. Williamson, 1975. The probability of the survival of a mutant gene in an age-structured population and implications for the evolution of life-histories. Genet. Res. 26: 1–10.
Cooley, L., R. Kelley & A. Sprading, 1988. Insertional mutagenesis of the Drosophila genome with single P-elements. Science 239: 1121–1128.
Cox, D. R., 1972. Regression models and life-tables (with discussion). J. Roy. Stat. Soc. B 34: 187–200.
Darnell, J., H. Lodish & D. Baltimore, 1990. Molecular cell biology. 2nd Ed. W. H. Freeman, New York.
Hamilton, W. D., 1966. The moulding of senescence by natural selection. J. Theor. Biol 12: 12–45.
Hillesheim, E. & S. C. Stearns, 1992. Correlated responses in life history traits to artificial selection for body weight inDrosophila melanogaster. Evolution 46: 745–52.
Hovemann, B., S. Richter, U. Walldorf & C. Cziepluch, 1988. Two genes encode related cytoplasmic elongation factors EF-1a inDrosophila melanogaster with continuous and stage specific expression. Nucleic Acids Res. 16: 3175–94.
Johnson, T. E., 1990. Increased life-span ofage-1 mutants inCaenorhabditis elegans and lower Gompertz rate of aging. Science 249: 908–912.
Kaiser, M. & S. C. Stearns,1993. Effects on fitness components of enhanced expression of elongation factor EF-1α inDrosophila melanogaster II. Genetic backgrounds and insert positions. Under review.
Luckinbill, L., R. Arking, M. G. Clare, W. C. Cirocco & S. A. Buck, 1984. Selection for delayed senescence inDrosophila melanogaster. Evolution 38: 996–1003.
Partridge, L. & K. Fowler, 1992. Direct and correlated responses to selection for age at reproduction inDrosophila melanogaster. Evolution 46: 76–91.
Robertson, H. M., C. R. Preston, R. W. Phillis, D. M. Johnson-Schlitz, W. K. Benz & W. R. Engels, 1988. A stable source of P-element transposase inDrosophila melanogaster. Genetics 118: 61–470.
Rose, M., 1984. Laboratory evolution of postponed senescence inDrosophila melanogaster. Evolution 38: 1004–1010.
Rose, M. R., 1991. Evolutionary biology of aging. oxford University Press, Oxford.
Rose, M. R. & B. Charlesworth, 1980. A test of evolutionary theories of senescence. Nature 287: 141–142.
Rose, M. R. & B. Charlesworth, 1981. Genetics of life history inDrosophila melanogaster. II. Exploratory selection experiments. Genetics 97: 187–196.
SAS Institute, 1985. SAS User's Guide: Statistics. SAS Institute, Cary, North Carolina, 957 p.
SAS Institute, 1991. SAS Technical report P-127. SAS/STAT Software: The PHREG procedure. Version 6. SAS Institute, Cary, North Carolina. 59 p.
Shepherd, J. C. W., U. Walldorf, P. Hug & W. J. Gehring, 1989. Fruit flies with additional expression of the elongation factor EF-1α life longer. Proc. Natl. Acad. Sci. USA 86: 7520–1.
Sokol, R. R. & F. J. Rohlf, 1981. Biometry. 2nd Ed. W. H. Freeman, San Francisco.
Stearns, S. C., T. Diggelmann, M. Gebhardt, H. Bachmann & R. Wechsler, 1987. A. device for collecting flies of precisely determined post-hatching age. Drosophila Information Service 66: 167–169.
Stearns, S. C., 1992. The evolution of life histories. Oxford University Press, London, 248 p.
Stearns, S. C., M. Kaiser & E. Hillesheim, 1993. Effect on fitness components of enhanced expression of elongation factor EF-1α inDrosophila melanogaster. I. The contrasting approaches of molecular and population biologists. Am. Nat. (in press).
Stearns, S. C. & M. Kaiser, 1993. Effects on fitness components of enhanced expression of elongation factor EF-1α inDrosophila melanogaster. III. Analysis of tradeoffs between fitness components. Under review.
Webster, G. C. & S. L. Webster, 1982. Effects of age on the post-initiation stages of protein synthesis. Mech. Aging Dev. 18: 369–378.
Webster, G. C. & S. L. Webster, 1983. Decline in synthesis of elongation factor on (EF-1) precedes the decreased synthesis of total protein in agingDrosophila melanogaster. Mech. Aging Dev. 22: 121–128.
Williams, G. C., 1957. Pleiotropy, natural selection, and the evolution of senescence. Evolution 11: 398–411.
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Stearns, S.C., Kaiser, M. The effects of enhanced expression of elongation factor EF-1α on lifespan inDrosophila melanogaster . Genetica 91, 167–182 (1993). https://doi.org/10.1007/BF01435996
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DOI: https://doi.org/10.1007/BF01435996