Summary
Sephadex G 200 chromatography of non-contacted human or bovine plasma reveals one peak of kininogen (LMW kininogen) which serves as substrate for both trypsin and contact kallikrein (= prekallikrein activated by Hageman Factor.)
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2.
Under special conditions (faster flow rate, protection with diisopropyl fluorophosphate and hexadimethrine bromide), a small kininogen fraction of higher molecular weight (HMW) occasionally preceded the LMW substrate. It appeared increased when the plasma was hemolytic whether contacted or not.
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3.
Native human plasma was subjected to DEAE sephadex chromatography using stepwise elution starting with relatively high salt concentration. The second peak called HMW-kininogen emerged together with the abrupt increase in ionic strength and contained 2.2 to 4.8 times less kinin than did the first. Both kininogens yielded kinin with purified contact kallikrein.
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4.
By analytical gel filtration of different HMW-kininogen preparations, molecular weights of about 100,000, 200,000 and 500,000 were estimated.
We conclude that HMW-kininogen is not represented by a distinct, individual molecule. It may be a polymer of the low molecular weight kininogen or an aggregate of it with other plasma constituents.
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References
Andrews, P.: The gel-filtration behaviour of proteins related to their molecular weights over a wide range. Biochem. J.96, 595–606 (1965).
Armstrong, D., Mills, G. L., Sieuteri, F.: Physiological influence on the liberation of human plasma kinin at low temperatures. In E. G. Erdös, N. Back, and F. Sicuteri: Hypotensive Peptides, pp. 139–148. Berlin-Heidelberg-New York: Springer 1966.
Diniz, C. R., Carvalho, J. F.: A micro method for determination of bradykininogen under several conditions. Ann. N. Y. Acad. Sci.104, 77–89 (1963).
Gautvik, K.: Kininogen in plasma from cat, hamster and guinea pig. Acta physiol. scand.76, 191–200 (1969).
Habermann, E.: Über pH-bedingte Modifikationen des kininlieferndenα-Globulins (Kininogen) aus Rinderserum und das Molekulargewicht von Kininogen I. Biochem. Z.337, 440–448 (1963).
—: Strukturaufklärung kininliefernder Peptide aus Rinderserumkininogen. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak.253, 474–483 (1966).
—: Kininogens. In Handbook of Experimental Pharmacology, vol. 25, pp. 250–288. Berlin-Heidelberg-New York: Springer 1970.
—, Klett, W.: Reinigung und einige Eigenschaften eines Kallikreins aus Schweineserum. Biochem. Z.346, 133–158 (1966).
Horton, E. W.: The assay of kallikrein in urine. J. Physiol. (Lond.)142, 36P (1958).
Jacobsen, S.: Substrates for plasma kinin forming enzymes in human, dog and rabbit plasmas. Brit. J. Pharmacol.26, 403–411 (1966).
—, Kriz, M.: Some data on two purified kininogens from human plasma. Brit. J. Pharmacol.29, 25–36 (1967).
Jahrreiss, R., Habermann, E.: In vitro investigations on some components of the kinin system (kininogen, serum kallikrein, Hageman factor) and their interaction. In F. Sicuteri (Ed.): Bradykinin and related kinins: Ccardiovascular, biochemical, and neural actions. New York-London: Plenum Press 1970.
— —: On the endogenous mechanism of kinin release. I. Attempts at discriminating kallikreins. Naunyn-Schmiedebergs Arch. Pharmak.269, 85–100 (1971).
Kato, H., Nagasawa, S., Suzuki, T.: Studies on the structure of bovine kininogen: Cleavage of disulfide bonds and of methionyl bonds in kininogen II. J. Biochem.67, 313–323 (1970).
—, Suzuki, T.: The location and nature of the linkage of kinin moiety in bovine kininogen-II. Biochem. biophys. Res. Commun.32, 800–805 (1968).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the folin reagent. J. biol. Chem.193, 265–275 (1951).
Pierce, J. V.: Structural features of plasma kinins and kininogens. Fed. Proc.27, 52–57 (1968).
Temme, H., Jahrreiss, R., Habermann, E., Zilliken, F.: Aktivierung von Gerinnungs- und Kininsystem durch eine Plasma-Esterase (Hageman-Faktor). Reinigung und Wirkungsbedingungen. Hoppe-Seylers Z. physiol. Chem.350, 519–521 (1969).
Yano, M., Nagasawa, S., Horiuchi, K., Suzuki, T.: Separation of a new substrate, kininogen-I, for plasma kallikrein in bovine plasma. J. Biochem.62, 504–506 (1967).
— —, Suzuki, T.: Evidence for the location of methionyl-lysyl-bradykinin moieties at the carboxyl terminus and inside of bovine kininogen I. Biochem. biophys. Res. Commun.40, 914–918 (1970a).
— — —: Purification and properties of bovine serum kallikrein activated with casein. J. Biochem.67, 713–725 (1970b).
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Habermann, E., Jahrreiss, R. On the endogenous mechanism of kinin release. Naunyn-Schmiedebergs Arch. Pharmak. 269, 101–111 (1971). https://doi.org/10.1007/BF01422019
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DOI: https://doi.org/10.1007/BF01422019