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Acta Neurochirurgica

, Volume 133, Issue 3–4, pp 184–190 | Cite as

Effects of vitamin D and retinoic acid on human glioblastoma cell lines

  • L. Magrassi
  • G. Butti
  • S. Pezzotta
  • L. Infuso
  • G. Milanesi
Experimental Research

Summary

The biological significance of vitamin D receptors expressed by glioblastoma and other glial tumours is still unclear. In an effort to clarify this issue we studied the effects of increasing concentrations of 25-dihydroxyvitamin D3 and its metabolite 1 α, 25-dihydroxyvitamin D3 on two human glioblastoma cell lines. Both substances were capable of inducing a significant (> 50%) reduction in growth of the two glioblastoma cell lines at dosages over 5 μM. When the HU 70 cell line was treated by increasing dilutions of 25-dihydroxyvitamin D3 combined with 1 μM all trans-retinoic acid, significant inhibition was apparent even after addition of 25-dihydroxyvitamin D3 in the nanomolar range. Reduction of growth index was mainly due to induced cell death.

Our results providein vitro evidence that vitamin D metabolites alone or in combination with retinoids may be potentially useful agents in the differentiation therapy of human malignant gliomas.

Keywords

Glioblastoma retinoic acid vitamin D cell death 

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • L. Magrassi
    • 1
    • 2
  • G. Butti
    • 1
  • S. Pezzotta
    • 1
  • L. Infuso
    • 1
  • G. Milanesi
    • 2
  1. 1.Department of Surgery, Section of NeurosurgeryUniversity of PaviaPaviaItaly
  2. 2.IGBE CNRPaviaItaly

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