To evaluate the role of interleukin-8 (IL-8) on the activation of neutrophils in rheumatoid arthritis (RA), we measured IL-8 and the adhesion molecules, L-selectin (CD62L), CD11b and CD18, on neutrophils in paired peripheral blood and synovial fluid of RA patients. Synovial fluid IL-8 levels were significantly increased compared to peripheral blood. L-selectin was split off and CD 11b and CD18 were upregulated on neutrophils in the synovial fluid. A positive correlation occurred between the IL-8 concentration and CD18 or CD11b densities on neutrophils in the synovial fluid (r=0.75,P <0.005 andr=0.60,P < 0.05, respectively). Peripheral blood neutrophils of the patients were desensitised with IL-8 in vivo, as shown by the significantly lower L-selectin shedding after in vitro IL-8 stimulation: 1.6 times decrease for patients vs 3.2 for controls (P < 0.05). In conclusion, these results add further evidence for the role of IL-8 in the activation of neutrophils in RA.
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Endo H, Akahoshi T, Takagishi K, Kashiwazaki S, Matsushima K (1991) Elevation of interleukin-8 (IL-8) levels in joint fluids of patients with rheumatoid arthritis and the induction by IL-8 of leukocyte infiltration and synovitis in rabbit joints. Lymphokine Cytokine Res 10:245–252
Deleuran B, Lemche P, Kristensen M, Chu CQ, Field M, Jensen J, Matsushima K, Stengaard-Petersen K (1994) Localisation of interleukin 8 in the synovial membrane, cartilage-pannus junction and chondrocytes in rheumatoid arthritis. Scand J Rheumatol 23:2–7
Endo H, Akahoshi T, Nishimura A, Tonegawa M, Takagishi K, Kashiwazaki S, Matsusuhima K, Kondo H (1994) Experimental arthritis induced by continuous infusion of IL-8 into rabbit knee joints. Clin Exp Immunol 96:31–35
Springer TA (1990) Adhesion receptors of the immune system. Nature 346:425–433
Johnston JA, Ferris DK, Wang JM, Longo DL, Oppenheim JJ, Kelvin DJ (1994) Staurosporine restores signaling and inhibits interleukin-8-induced chemotactic desensitization. Eur J Immunol 24:2556–2562
Smith WB, Gamble JR, Clark-Lewis I, Vadas MA (1993) Chemotactic desensitization of neutrophils demonstrates interleukin-8 (IL-8) dependent and IL-8-independent mechanisms of transmigration through cytokine-activated endothelium. Immunology 78:491–497
Ropes MW, Bennett GA, Caleb S, Jacox R, Jessar RA (1958) Revision of diagnostic criteria for rheumatoid arthritis. Bull Rheum Dis 9:175–176
Ritchie DM, Boyle JA, McInnes JM (1968) Clinical studies with an articular index for the assessment of joint tenderness in patients with rheumatoid arthritis. Q J Med 37:393–406
Moens MM, Bridts CH, De Clerck LS, Van Bever HP, Stevens WJ (1992) Detection of hypodense eosinophils by Percoll multilayer gradient centrifugation in subjects with normal and slightly elevated eosinophilia. J Immunol Methods 156:271–275
Mertens AC, De Clerck LS, Moens MM, Bridts CH, Stevens WJ (1993) Lymphocyte activation status, expression of adhesion molecules and adhesion to human endothelium in rheumatoid arthritis — relationship to disease activity. Res Immunol 144:101–108
Schmid J, Schmid P, Giorgi JV (1988) Conversion of logarithmic channel numbers into relative linear fluorescence intensity. Cytometry 9:533–538
Rampart M, Herman AG, Grillet B, Opdenakker G, Van Damme J (1992) Development and application of a radioimmunoassay for interleukin-8: detection of interleukin-8 in synovial fluids from patients with inflammatory joint disease. Lab Invest 66:512–518
Baggiolini M, Clark-Lewis I (1992) Interleukin-8, a chemotactic and inflammatory cytokine. FEBS Lett 307:97–101
Strieter RM, Koch AE, Antony VB, Fick RB, Standiford TJ, Kunkel SL (1994) The immunopathology of chemotactic cytokines: the role of interleukin-8 and monocyte chemoattractant protein-1. J Lab Clin Med 123:183–197
Verburgh CA, Hart MHL, Aarden LA, Swaak AJG (1993) Interleukin-8 (IL-8) in synovial fluid of rheumatoid and nonrheumatoid joint effusions. Clin Rheumatol 12:494–499
Zimmerman GA, Prescott SM, McIntyre TN (1992) Endothelial cell interactions with granulocytes: tethering and signaling molecules. Immunol Today 13:93–100
Norgauer J, Krutmann J, Dobos GJ, Trayner-Kaplan AE, Oades ZG, Schraufstätter I (1994) Actin polymerization, calcium-transients, and phospholipid metabolism in human neutrophils after stimulation with interleukin-8 and formyl N-peptide. J Invest Dermatol 102:310–314
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De Gendt, C.M., De Clerck, L.S., Bridts, C.H. et al. Relationship between interleukin-8 and neutrophil adhesion molecules in rheumatoid arthritis. Rheumatol Int 16, 169–173 (1996). https://doi.org/10.1007/BF01419730
- CD 11b
- CD 18
- rheumatoid arthritis