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Myosin-ATPase fibre typing of chemically skinned muscle fibres

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Summary

The efficacy of myosin (M)-ATPase fibre typing to differentiate fibre types in chemically (EGTA) skinned muscle fibres was investigated. Cryosections or single fibres from isolated bundles of chemically skinned rat extensor digitorum longus (EDL) and soleus (SOL) muscles were stained for M-ATPase activity. The results indicate that two major fibre types (type I and II, Brooke & Kaiser, 1970) can be indentified, as well as subgrouping of the type II fibres into types IIa and IIb. Thus, chemically skinning muscle fibres appears to have no detrimental effects on subsequent M-ATPase fibre typing.

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Authors and Affiliations

  1. Muscle Biology Laboratory, School of Medicine, University of Wisconsin, 53706, Madison, Wisconsin, USA

    Thomas J. Eddinger & Robert G. Cassens

  2. Department of Physiology, School of Medicine, University of Wisconsin, 53706, Madison, Wisconsin, USA

    Richard L. Moss

Authors
  1. Thomas J. Eddinger
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  2. Richard L. Moss
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  3. Robert G. Cassens
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Eddinger, T.J., Moss, R.L. & Cassens, R.G. Myosin-ATPase fibre typing of chemically skinned muscle fibres. Histochem J 17, 1021–1026 (1985). https://doi.org/10.1007/BF01417950

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  • DOI: https://doi.org/10.1007/BF01417950

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