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Immunohistochemical staining of macrophages in the skin lesions of leprosy: the role of antibody to mycobacteria in human serum and various polyclonal immune rabbit antisera

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Summary

Immunohistochemical staining of tuberculoid and lepromatous leprosy skin lesions was performed using various rabbit antisera. Macrophages in both stained with serum containing antibodies against lysozyme and alpha-1-antitrypsin, while macrophages in lepromatous leprosy also reacted with other antibodies. An immunoglobulin fraction of positive serum stained following pepsin digestion, indicating that reactivity was not Fc dependent. Positive serum contained antibody againstMycobacterium butyricum, which caused macrophage staining, since affinity-purified antibody did not stain and absorption withM. butyricum removed staining. Staining was also produced by serum of subjects with leprosy or a positive tuberculin test. By immunoblotting, the anti-mycobacterial antibody was directed against surface components ofM. butyricum of molecular weights 20 000–70 000. Electron microscopy showedM. leprae in phagolysosomes of macrophages, while immunoelectron microscopy demonstrated labelling along bacterial cell membranes. Therefore, macrophages in lepromatous leprosy skin lesions stain because they containM. leprae, which reacts with antibody to eitherM. leprae, M. tuberculosis or atypical mycobacteria in human serum and with antibody toM. butyricum in serum from rabbits immunized with various antigens and Freund's complete adjuvant. These results indicate that immunohistochemical studies on leprosy are misleading if performed using intact polyclonal immune sera rather than affinity purified or monoclonal antibodies.

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Authors and Affiliations

  1. Department of Pathology, The Hospital for Sick Children, 555 University Avenue, M5G 1X8, Toronto, Ontario, Canada

    Harriette J. Kahn, Paul Thorner, Reuben Baumal & Herman Yeger

  2. Department of Pathology, Women's College Hospital, 76 Grenville St, M5S 1B2, Toronto, Canada

    Harriette J. Kahn & Lynn From

  3. Department of Pathology, Toronto General Hospital, 101 College St, M5G 1L7, Toronto, Canada

    Dennis Bailey

  4. Department of Pathology, Banting and Best Department of Medical Research, University of Toronto, 100 College St, M5G 1L5, Toronto, Canada

    Alexander Marks

  5. Department of Dermatology, The Wellesley Hospital, M4Y 1J3, Toronto, Ontario, Canada

    Benjamin K. Fisher & Charles Lynde

Authors
  1. Harriette J. Kahn
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  2. Paul Thorner
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  3. Reuben Baumal
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  4. Herman Yeger
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  5. Dennis Bailey
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  6. Alexander Marks
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  7. Lynn From
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  8. Benjamin K. Fisher
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  9. Charles Lynde
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Kahn, H.J., Thorner, P., Baumal, R. et al. Immunohistochemical staining of macrophages in the skin lesions of leprosy: the role of antibody to mycobacteria in human serum and various polyclonal immune rabbit antisera. Histochem J 17, 1009–1020 (1985). https://doi.org/10.1007/BF01417949

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  • DOI: https://doi.org/10.1007/BF01417949

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