Abstract
Brillouin scattering experiments have been performed on very dilute solutions of bovine serum albumin (BSA) in pH=4.7,I=0.1 M NaCl; insulin in pH=7.5,I = 0.1 M NaCl and polystyrene latex suspensions in aqueous medium at room temperatureT = 201 °C. The samples were first characterized by quasi-elastic light scattering (QELS) to determine the translational diffusion coefficient (D T), hydrodynamic radius (R H), and polydispersity of each of the samples at normal pressure. Subsequently, the samples were subjected to Brillouin scattering studies where the spectrum of the scattered light was analyzed by using a piezo-electric scannend five-pass Fabry-Perot interferometer. The hydrostatic pressure (P) acting on the samples was varied in the range from 1B to 825 B in steps of 100 B each. The pressure dependence of the measured sound velocityV s (P) could be least square fitted tov s (P)=A0 +A 1 P +A 2 P 2 with experimental error (± 1 %). This data has been interpreted consistent with an earlier work within the framework of modified Tait's equation of state and presumption of linear pressure dependence of the adiabatic bulk modulus in the moderate pressure regime.
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Bohidar HB (1988) J Appl Phys 64:1810
Bohidar HB (1988) J Appl Phys 64:3766
Nystråm B, Roots J (1983) J Chem Phys 78:2833
Roots J, Nystråm B (1982) Macromolecules 15:553
Bohidar H, Geissler E (1984) Biopolymers 23:2407
Berne BJ, Pecora R (1976) Dynamic light scattering. Wiley-Interscience, New York; Chu B (1974) Laser light scattering. Academic, New York
Fleury PA (1973) Adv Chem Phys 24:1
Bohidar HB, Berland T, Feder J, Jøassang T (1987) Rev Sci Instrum, 58:1422
McDonald JR (1966) Rev Mod Phys 38:669
Anderson OL (1966) J Phys Chem Solids 27:547
Zoller P (1978) J Appl Polym Sci 22:633, ibid (1979) 23:1051, 1057; ibid (1977) 21:3129
Quach A, Simha R (1971) J Appl Phys 42:4592
Beret S, Prausnitz JM (1975) Macromolecules 8:536
Runincio JAR, Prausnitz JM (1976) Macromolecules 9:524
Quach A, Wilson PS (1974) J Macromol Sci Phys B-9:533
Dil JG (1982) Rep Prog Phys 45:285
Koppel DE (1972) J Chem Phys 57:4814
Bohidar HB, Berland T, Boger F, Jøssang T, Feder J (1987) Department of Physics Rep 87-09, University of Oslo, Norway (available on request from author)
Berland T, Bohidar H, Jøssang T, Feder J (1986) Department of Physics Rep 86-18, University of Oslo, Norway (available on request from authors)
Morse PM, Ingrad KU (1968) Theoretical Acoustics. Mc-Graw Hill, New York
Pusey PN (1974) In: Cummins HZ, Pike ER (eds) Photon correlation and light beating spectroscopy. Plenum, New York
(1975/76) Handbook of Chemistry and Physics. Vol 56, CRC Press, USA
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Bohidar, H.B. Light scattering study of solution properties of bovine serum albumin, insulin, and polystyrene under moderate pressure. Colloid & Polymer Sci 267, 292–300 (1989). https://doi.org/10.1007/BF01413622
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DOI: https://doi.org/10.1007/BF01413622