Abstract
The adsorbability of bovine serum albumin (BSA) onto styrene/acrylic acid copolymer (PS/PAA) latex prepared without emulsifier was investigated as a function of pH and ionic strength. Polystyrene (PS) latex was used as a reference sample. The adsorption isotherms of BSA onto PS/PAA latices showed a stepwise nature. The thickness of the adsorbed BSA monolayer suggested that BSA molecules adsorbed onto PS/PAA2 latex (here, the subscript, i. e., 2, represents the mol % of acrylic acid used in copolymerization) in a “side-on” mode near the isoelectric point (IEP) of this protein. However, in the case of PS/PAA5 latex, a portion of the BSA molecules probably adsorbed in a “loop” or an “end-on” mode. The amount of BSA adsorbed onto PS/PAA latices greatly depended on pH and ionic strength; its value showed a maximum in the neighborhood of the IEP of BSA as in PS latex. The amount adsorbed onto PS/PAA latices except in the alkaline pH region was greater than that onto PS latex. Moreover, the amount adsorbed onto PS/PAA5 latex was greater than that onto PS/PAA2 latex. Both these results are probably attributed to hydrogen bonding between BSA molecule and PS/PAA latex. It was found that the adsorbability of BSA especially at a high ionic strength greatly depended on the surface properties of polymer latices.
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Kim SW, Lee RG, Oster H, Lentz D, Coleman L, Andrade JD, Olsen D (1974) Trans Am Soc Artif Int Organs 20:449
Norde W (1976) Thesis Ph D, Agricultural University Wageningen, The Netherlands
Okano T, Nishiyama S, Shinohara I, Akaike T, Sakurai Y (1979) Kobunshi Ronbunshu 36:209
Fair BD, Jamieson AM (1980) J Coll Interf Sci 77:525
Suzawa T, Murakami T (1980) J Coll Interf Sci 78:266
Suzawa T, Shirahama H, Fujimoto T (1982) J Coll Interf Sci 86:144
Suzawa T, Shirahama H, Fujimoto T (1983) J Coll Interf Sci 93:498
Shirahama H, Suzawa T, J Coll Interf Sci, in press
Shirahama H, Suzawa T (1984) Polym J 16:795
Matsumoto T, Shimada M (1965) Kobunshi Kagaku 22:172
Shirahama H, Suzawa T (1984) J Appl Polym Sci, in press
Itzhaki RF, Gill DM (1964) Anal Biochem 9:401
Kim SW, Lee RG (1975) (ed) Baier RE, Applied Chemistry at Protein Interfaces, Am Chem Soc, New York pp 218–229
Chiu T-H, Nyilas E, Lederman DM (1976) Trans Am Soc Artif Int Organs 22:498
Hlady V, Füredi-Milhofer H (1979) J Coll Interf Sci 69:460
Williams EJ, Foster JF (1959) J Am Chem Soc 81:865
Markus G, Karush F (1957) J Am Chem Soc 79:3264
Leonard Jr WJ, Vijai KK, Foster JF (1963) J Biol Chem 238:1984
Seno M (ed) (1978) Iyo Kobunshi, Kyoritsu Shuppan, Tokyo, p 76
Ikada Y, Iwata H, Horii F, Matsunaga T, Taniguchi M, Suzuki M, Taki W, Yamagata S, Yonekawa Y, Handa H (1981) J Biomed Mater Res 15:697
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Shirahama, H., Suzawa, T. Adsorption of bovine serum albumin onto styrene/acrylic acid copolymer latex. Colloid & Polymer Sci 263, 141–146 (1985). https://doi.org/10.1007/BF01412788
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DOI: https://doi.org/10.1007/BF01412788