Summary
A method utilizing centrifugal elutriation to obtain a highly purified (95 ± 2% after 3 d in culture) preparation of beating cardiac myocytes from enzyme-dissociated neonatal rat heart is described. An additional fraction enriched for nonmuscle cells is also obtained. Purity of myocyte cultures is determined by Coomassie blue R250 staining of the cytoskeleton; this procedure is also described.
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Ulrich, R.G., Elliget, K.A. & Rosnick, D.K. Purification of neonatal rat cardiac cells by centrifugal elutriation. Journal of Tissue Culture Methods 11, 217–221 (1988). https://doi.org/10.1007/BF01407317
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DOI: https://doi.org/10.1007/BF01407317