Summary
Co-transformation experiments were carried out onPetunia hybrida protoplasts. The method used was electroporation with two plasmids: one confering kanamycin resistance, and the other harbouring a phosphoenolpyruvate carboxylase (PEPC) cDNA fromSorghum vulgare leaves. Southern blot analysis of the selected lines demonstrated a high co-transformation frequency.
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Abbreviations
- PEPC:
-
phosphoenolpyruvate carboxylase
- NPT:
-
neomycin phosphotransferase
- ATF:
-
absolute transformation frequency
- PEG:
-
polyethylenglycol
- BA:
-
6-benzyl-aminopurine
- IAA:
-
3-indoleacetic acid
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
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Tagu, D., Bergounioux, C., Cretin, C. et al. Direct gene transfer inPetunia hybrida electroporated protoplasts: Evidence for co-transformation with a phosphoenolpyruvate carboxylase cDNA from sorghum leaf. Protoplasma 146, 101–105 (1988). https://doi.org/10.1007/BF01405918
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DOI: https://doi.org/10.1007/BF01405918