Summary
Basal forebrain cells of foetal rats were transplanted into the subarachnoid space of adult rats harbouring a kainic acid-induced unilateral lesion in the nucleus basalis magnocellularis. Passive avoidance response tests were performed eight weeks after the transplantation, and the results were compared with those of lesioned but non-transplanted rats and of non-lesioned control rats.
Although acquisition impairments did not improve, retention impairments were significantly ameliorated in the transplanted rats. Histologically, transplanted foetal neurons survived and grew very well over the cortical surface, and exhibited facilitated neuritic elongation on acetylcholinesterase staining. Choline acetyl-transferaseimmunoreactive neurons were found along the needle track as well as in the subarachnoid graft tissues.
The results seem to indicate that not the re-innervation from the graft to the host cortex but the diffusional supply of neurotransmitters and/or their synthetic enzymes and neurotrophic factors were responsible for improvement of memory deficits. The subarachnoid space proved to be an adequate place for growth of transplanted neuronal and glial cells for reasons of ample supply of oxygen and nutrition and of low tissue pressure.
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References
Davies P, Maloney AJF (1976) Selective loss of central cholinergic neurons in Alzheimer's disease. Lancet ii: 1403
Dunnett SB, Low WC, Iversen SD, Stenevi U, Björklund A (1982) Septal transplants restore maze learning in rats with fornix-fimbria lesions. Brain Res 251: 335–348
Dunnett SB, Toniolo G, Fine A, Ryan CN, Björklund A, Iversen SD (1985) Transplantation of embryonic ventral forebrain neurons to the neocortex of rats with lesions of nucleus basalis magnocellularis. II. Sensorimotor and learning impairments. Neuroscience 16: 787–797
Emmett CJ, Lawrence JM, Seeley PJ, Raisman G (1988) Studies of the behaviour of purified rat astrocytes after transplantation into syngeneic adult brain. Prog Brain Res 78: 383–386
Fine A, Dunnett SB, Björklund A, Iversen SD (1985) Cholinergic ventral forebrain grafts into the neocortex improve passive avoidance memory in a rat model of Alzheimer disease. Proc Natl Acad Sci USA 82: 5227–5230
Fine A, Dunnett SB, Björklund A, Clarke D, Iversen SD (1985) Transplantation of embryonic ventral forebrain neurons to the neocortex of rats with lesions of nucleus basalis magnocellularis. I. Biochemical and anatomical observations. Neuroscience 16: 769–786
Flicker C, Dean RL, Watkins DL, Fisher SK, Bartus RT (1983) Behavioral and neurochemical effects following neurotoxic lesions of a major cholinergic input to the cerebral cortex in the rat. Pharmac Biochem Behav 18: 973–981
Friedman E, Lerer B, Kuster J (1983) Loss of cholinergic neurons in the rat neocortex produces deficits in passive avoidance learning. Pharmacol Biochem Behav 19: 309–312
Furukawa S, Furukawa Y, Satoyoshi E, Hayashi K (1986) Synthesis and secretion of nerve growth factor by mouse astroglial cells in culture. Biochem Biophys Res Commun 136: 57–63
Hefti F (1986) Nerve growth factor (NGF) promotes survival of septal cholinergic neurons after fimbrial transections. J Neurosci 6: 2155–2162
Itakura T, Umemoto M, Kamei I, Imai H, Yokote H, Yukawa S, Komai N (1992) Autotransplantation of peripheral cholinergic neurons into the brains of Alzheimer model rats. Acta Neurochir (Wien) 115: 127–132
Kromer LF (1987) Nerve growth factor treatment after brain injury prevents neuronal death. Science 235: 214–216
Kyoshima K, Matsuda M, Handa J (1992) Growth of the graft and astrocytic reaction following transplantation of fetal brain to adult rat's brain. Part II. Cell suspension transplantation into the subarachnoid space. Arch Jpn Chir 61: 27–34
Lerer B, Warner J, Friedman E (1985) Cortical cholinergic impairment and behavioural deficits produced by kainic acid lesions of rat magnocellular basal forebrain. Behav Neurosci 99: 661–677
Logan A, Frautschy SA, Baird A (1991) Basic fibroblast growth factor and central nervous system injury. Ann NY Acad Sci 63: 474–476
Lynch GS, Lucas PA, Deadwyler SA (1972) The demonstration of acetylcholinesterase containing neurons within the caudate nucleus of the rat. Brain Res 45: 617–621
Perry EK, Perry PH, Blessed G, Tomlinson BE (1977) Necropsy evidence of central cholinergic deficits in senile dementia. Lancet i: 189
Tago H, Kimura H, Maeda T (1986) Visualization of detailed acetylcholinesterase fiber and neuron staining in rat brain by a sensitive histochemical procedure. J Histochem Cytochem 34: 1431–1438
White P, Hiley CR, Goodhardt MJ, Carrasco LH, Keet JP, Williams IEI, Bowen DM (1977) Neocortical cholinergic neurons in elderly people. Lancet i: 668–670
Whitehouse PJ, Price DL, Struble RG, Clark AW, Coyle JT, DeLong MR 1982) Alzheimer's disease and senile dementia: loss of neurons in the basal forebrain. Science 215: 1237–1239
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Kyoshima, K., Matsuda, M. & Handa, J. Transplantation of basal forebrain cells of foetal rats into the subarachnoid space: Improvement of disturbance of passive avoidance memory due to injury of nucleus basalis magnocellularis. Acta neurochir 133, 68–72 (1995). https://doi.org/10.1007/BF01404951
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DOI: https://doi.org/10.1007/BF01404951