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Processing of wild caughtCulicoides and isolation of bluetongue virus using a mosquito cell line

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Journal of tissue culture methods

Summary

Isolation of bluetongue virus from theCulicoides vector can provide valuable epidemiologic information. The methods described offer a practical procedure that ensures the preservation of the virus during field processing of the collection and an assay system that utilizes C6/36 cells, a clone derived fromAedes albopictus. The C6/36 cell line affords a sensitive isolation system and a practical virus detection system suitable for mass screening of insect pools.

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Authors and Affiliations

  1. Department of Pathology, School of Veterinary Medicine, University of California, 95616, Davis, California

    Mary Sawyer & Bennie I. Osburn

Authors
  1. Mary Sawyer
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  2. Bennie I. Osburn
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Sawyer, M., Osburn, B.I. Processing of wild caughtCulicoides and isolation of bluetongue virus using a mosquito cell line. Journal of Tissue Culture Methods 11, 171–173 (1988). https://doi.org/10.1007/BF01404271

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  • DOI: https://doi.org/10.1007/BF01404271

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