Summary
A method is described for the production of monolayer cultures of epithelial-like cells from fragments of fetal bovine spleen. The initial fibroblasticlike outgrowth from such fragments kept in stationary culture is soon replaced by epithelial-like cells which thereafter predominate. The latter, commonly referred to as BESp cells, have been useful for isolation, identification, and propagation of several bovine viruses.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Gonda, M. A.; Braun, M. J.; Carter, S. G., et al. Characterization and molecular cloning of bovine lentivirus related to human immunodeficiency virus. Nature 330:388–391; 1987.
Malmquist, W. A.; Van Der Maaten, M. J.; Boothe, A. D. Isolation, immunodiffusion, immunofluorescence, and electron microscopy of a syncytial virus of lymphosarcomatous and apparently normal cattle. Cancer Res. 29:188–200; 1969.
Mengeling, W. L.; Ridpath, J. F.; Vorwald, A. C. Size and antigenic comparisons among the structural proteins of selected autonomous parvoviruses. J. Gen. Virol. 69:825–837; 1988.
Van Der Maaten, M. J.; Boothe, A. D. Isolation of herpes-like virus from lymphosarcomatous cattle. Arch. Virusforschung 37:85–96; 1972.
Van Der Maaten, M. J.; Boothe, A. D.; Seger, C. L. Isolation of a virus from cattle with persistent lymphocytosis. JNCI 49:1649–1657; 1972.
Van Der Maaten, M. J.; Miller, J. M.; Boothe, A. D. Replicating type C virus particles in monolayer cell cultures of tissues from cattle with lymphosarcoma. JNCI 52:491–497; 1974.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mengeling, W.L., Van Der Maaten, M.J. Preparation of bovine embryonic spleen cell cultures. Journal of Tissue Culture Methods 11, 135–138 (1988). https://doi.org/10.1007/BF01404266
Issue Date:
DOI: https://doi.org/10.1007/BF01404266