Skip to main content
SpringerLink
Log in

Establishment, characteristics, and diagnostic uses of cell cultures derived from tissues of exotic species

  • Published:
Journal of tissue culture methods

Summary

Methods for the preparation, cultivation, preservation, and maintenance of serially passaged cell cultures derived from different tissues of exotic or threatened animals and certain species of birds are described. The diagnoses of viral pathogens affecting exotic and domestic species were enhanced by the availability of such cell or avian tracheal ring organ cultures to the veterinary diagnostic virology laboratory. Cell cultures from exotic species derived from tissues of bighorn sheep, white-tailed gnu, white-tailed deer, oryx, aoudad sheep, cockatiels, and ring-necked pheasants were developed or obtained by our laboratory. These cell cultures were examined for cellular morphology and growth characteristics at two different temperatures, presence of adventitious agents, and karyotype. Several of the exotic cell lines supported the replication of various viruses associated with avian and mammalian hosts. As comparison, cell cultures of domestic sheep were used. Tracheal ring organ cultures from chickens, turkeys, and pheasants were also assayed for their sensitivity to selected viruses. Cell cultures from exotic species may provide a viable alternative for the detection of viruses from such species which heretofore have been difficult to isolate using cell lines derived from domestic species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Acosta, D.; Sorensen, E. M. B.; Anuforo, D. C., et al. An in vitro approach to the study of target organ toxicity of drugs and chemicals. In Vitro 21:495–504; 1985.

    Google Scholar 

  2. Adler, E. M.; Fluk, L. J.; Mullin, J. M., et al. Anomalous patterns in cultured cell monolayers. Science 217:851–852; 1982.

    PubMed  Google Scholar 

  3. Castro, A. E.; Montague, S. R.; Dotson, J. F., et al. Replication of 5 serotypes of bluetongue virus in a fetal tongue cell line derived from bighorn sheep and its potential for the isolation of viruses. J. Vet. Diag. Invest.; In Press 1989.

  4. Castro, A. E.; Reynolds, B.; Hill, D., et al. Diagnosis of avian infectious bronchitis virus in clinical and field specimens using tracheal ring organ cultures and serology. Proc. Western Poultry Disease Conference 37:36–40; 1988.

    Google Scholar 

  5. Chen, T. R.In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain. Exp. Cell Res. 104:255–262; 1977.

    PubMed  Google Scholar 

  6. Cowen, B. S.; Braune, M. O. The propagation of avian viruses in a continuous cell line (QT35) of Japanese quail origin. Avian Dis. 32:282–297; 1988.

    PubMed  Google Scholar 

  7. Dotson, J. F.; Castro, A. E. A preliminary study of the number of chromosomes in cells from the white-tailed gnu (Connochaetes gnou). Zoo Sounds 10; 1982.

  8. Foster, N. M.; Luedke, A. J. Direct assay for bluetongue virus by intravascular inoculation of embryonating chicken eggs. Am. J. Vet. Res. 29:749–753; 1968.

    PubMed  Google Scholar 

  9. Fowler, M. E., ed. Zoo and wild animal medicine, 2nd ed. Philadelphia: W. B. Saunders Co.; 1986:1056–1067.

    Google Scholar 

  10. Freshney, R. I. Culture of animals cells—A manual of basic technique, 2nd ed. New York: Alan R. Liss, Inc.; 1987:1–12, 69–84, 107–136, 207–214, 245–256, 309–343.

    Google Scholar 

  11. Goldsmit, L.; Barzilai, E. An improved method for the isolation and identification of bluetongue virus by intravenous inoculation of embryonating chicken eggs. J. Comp. Pathol. 78:477–487; 1968.

    PubMed  Google Scholar 

  12. Hay, R. J., ed. ATCC quality control methods for cell lines, 1st ed. Rockville, MD: American Type Culture Collection; 1985:7–24.

    Google Scholar 

  13. Hay, R.; Macy, M.; Corman-Weinblatt, A., et al., eds. American Type Culture Collection catalogue of cell lines and hybridomas, 5th ed. Rockville, MD: American Type Culture Collection; 1985:287–293.

    Google Scholar 

  14. Hay, R. J.; Strehler, B. L. The limited growth span of cell strains isolated from the chick embryo. Exp. Gerontol 2:123–135; 1967.

    Google Scholar 

  15. Hayflick, L. The cell biology of human aging. Sci. Am. 242:58–65; 1980.

    PubMed  Google Scholar 

  16. Heuschele, W. P. Diagnosis of malignant catarrhal fever due to alcelaphine herpesvirus-1. Proceeding III Int. Sym. Vet. Lab. Diag.: 707–713; 1983.

  17. Heuschele, W. P. Infectious diseases. In: Fowler, M. E., ed. Zoo and wild animal medicine, 2nd ed. Philadelphia: W. B. Saunders Co.; 1986:58–62.

    Google Scholar 

  18. Hsu, T. C. Karyology of cells in culture. A. Preparation and analysis of karyotypes and idiograms. In: Kruse, P. F., Jr.; Patterson, M. K., eds. Tissue culture methods and applications. New York: Academic Press: 1973:764–767.

    Google Scholar 

  19. Jalal, S. M.; Markvong, A.; Hsu, T. C. Differential chromosomal fluorescence with 33258 Hoechst. Exp. Cell Res. 90:443–444; 1975.

    PubMed  Google Scholar 

  20. Lennette, E. H.; Schmidt, N. J. Future prospects of tissue culture in microbiology. In: Maramorosch, K.; Hirumi, H., eds. Practical tissue culture applications. New York: Academic Press; 1979:409–422.

    Google Scholar 

  21. Liendo, G.; Castro, A. E. Bluetongue in cattle: diagnosis and virus isolation. Bovine Pract. 16:87–95; 1981.

    Google Scholar 

  22. McGarrity, G. J. Sources of stable cell lines. In: Methods of enzymology, vol. 58. New York: Academic Press; 1979:439–444.

    Google Scholar 

  23. Murrell, L. R. Vertebrate cell culture: an overview. In: Maramorosch, K.; Hirumi, H., eds. Practical tissue culture applications. New York: Academic Press; 1979;9–25.

    Google Scholar 

  24. Nelson-Rees, W. A.; Daniels, D. W.; Flandermeyer, R. R. Cross-contamination of cells in culture. Science 212:446–452; 1981.

    PubMed  Google Scholar 

  25. Nuzzolo, L.; Vellucci, A. Tissue culture techniques. St. Louis, MO: Warren H. Green, Inc.; 1983:75–116, 194–231.

    Google Scholar 

  26. Paul, J. Cell and tissue culture, 5th ed. New York: Churchill Livingstone Publishers; 1975:31, 47, 189–252.

    Google Scholar 

  27. Roberts, S. J. Veterinary obstetrics and genital diseases (theriogenology). Ann Arbor, Michigan: Edward Brothers; 1971:92.

    Google Scholar 

  28. Schaeffer, W. I. Usage of vertebrate, invertebrate, and plant cell, tissue and organ terminology. In Vitro 20:19–24; 1984.

    PubMed  Google Scholar 

  29. Yilma, T. Sensitivity of ovine choroid plexus cells to human and other animal interferons. J. Gen. Virol. 64:2013–2016; 1983.

    PubMed  Google Scholar 

  30. Zoological Society of San Diego; Necropsy report #24699; Jan. 13, 1988.

Download references

Author information

Authors and Affiliations

  1. California Veterinary Diagnostic Laboratory System, Virology Laboratory, University of California, 95616, Davis, California

    Jill F. Dotson & Anthony E. Castro

Authors
  1. Jill F. Dotson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Anthony E. Castro
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dotson, J.F., Castro, A.E. Establishment, characteristics, and diagnostic uses of cell cultures derived from tissues of exotic species. Journal of Tissue Culture Methods 11, 113–121 (1988). https://doi.org/10.1007/BF01404263

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01404263

Key words

Search

Navigation