Summary
Techniques have been established for the generation of bovine cytotoxic T cell lines and clones specific for lymphocytes infected with the protozoan parasiteTheileria parva. Theileria-specific cytotoxic T cell lines are generated by repeated stimulation in vitro with autologousT. parva-infected cells, of peripheral blood mononuclear cells from cattle immunized withT. parva. Theileria-specific cytotoxic T cell clones can be derived from these restimulated cultures by limiting dilution of the cells in the presence of irradiated stimulator and filler cells and T cell growth factor. The clones have the BoT4− BoT8+ phenotype and are restricted by class I MHC products. Parasite strain specificity of the clones differed depending on the parasite stock used for immunization, and in some instances differed between individual animals immunized with the same parasite stock. Preliminary evidence suggests that the latter is due to an influence of the MHC phenotype of the animal. Results of the parasite strain specificity of the cytotoxic T cell response are consistent with findings of cross-immunization experiments with the two stocks of the parasite studied.
This is a preview of subscription content, access via your institution.
References
Baldwin, C. L.; MacHugh, N. D.; Ellis, J. A., et al. Monoclonal antibodies which react with bovine T-lymphocyte antigens and induce blastigenesis: tissue distribution and functional characteristics of the target antigens. Immunology 63: 439–446; 1988.
Baldwin, C. L.; Morrison, W. I.; Naessens, J. Differentiation antigens and functional characteristics of bovine leukocytes. In: Miyasaka, M.; Trnka, Z. eds. Differentiation antigens in lymphohemopoietic tissues. New York and Basel: Marcel Dekker, Inc.; 1988:455–498.
Baldwin, C. L.; Teale, A. J.; Naessens, J., et al. Characterization of a subset of bovine T lymphocytes that express BoT4 by monoclonal antibodies and function: similarity to lymphocytes defined by human T4 and murine L3T4. J. Immunol. 136:4385–4391; 1986.
Brodsky, F. M.; Stone, W. H.; Parham, P. Of cows and men: a comparative study of histocompatibility antigens. Hum. Immunol. 3:143–152; 1981.
Buus, S.; Sette, A.; Colon, S. M., et al. The relation between major histocompatibility complex (MHC) restriction and the capacity of Ia to bind immunogenic peptides. Science 235:1353–1358; 1987.
Ellis, J. A.; Baldwin, C. L.; MacHugh, N. D., et al. Characterization by a monoclonal antibody and functional analysis of a subset of bovine T lymphocytes that express BoT8, a molecule analogous to human CD8. Immunology 58:351–358; 1986.
Emery, D. L.; Eugui, E. M.; Nelson, R. T., et al. Cell-mediated immune responses toTheileria parva (East Coast fever) during immunization and lethal infections in cattle. Immunology 43:323–336; 1981.
Eugui, E. M.; Emery, D. L. Genetically restricted cell-mediated cytotoxicity in cattle immune toTheileria parva. Nature 290:251–254; 1981.
Goddeeris, B. M.; Morrison, W. I.; Teale, A. J. Generation of bovine cytotoxic cell lines, specific for cells infected with the protozoan parasiteTheileria parva and restricted by products of the major histocompatibility complex. Eur. J. Immunol. 16:1243–1249; 1986.
Goddeeris, B. M.; Morrison, W. I.; Teale, A. J., et al. Bovine cytotoxic T-cell clones specific for cells infected with the protozoan parasiteTheileria parva: parasite strain specificity and class I major histocompatibility complex restriction. Proc. Natl. Acad. Sci. USA 83:5238–5242; 1986.
Henry, C.; Marbrook, J.; Vann, D. C., et al. Limiting dilution analysis. In: Mishell, B. B.; Shiggi, S. M., eds. Selected methods in cellular immunology. San Fracisco: W. H. Freeman & Co.; 1980:138–152.
Irvin, A. D.; Dobbelaere, D. A. E. Mwamachi, D. M., et al. Immunisation against East Coast fever: correlation between monoclonal antibody profiles ofTheileria parva stocks and cross immunity in vivo. Res. Vet. Sci. 35:341–346; 1986.
Kemp, S. J.; Spooner, R. L.; Teale, A. J. A comparative study of major histocompatibility complex antigens in East African and European cattle breeds. Animal Genet. 19:17–29; 1988.
Kurtti, T. J.; Munderloh, U. G.; Irvin, A. D., et al.Theileria parva: early events in the development of bovine lymphoblastoid cell lines peristently infected with macroschizonts. Exp. Parasitol. 52:280–290; 1981.
Lalor, P. A.; Morrison, W. I.; Goddeeris, B. M., et al. Monoclonal antibodies identify phenotypically and functionally distinct cell types in the bovine lymphoid system. Vet. Immunol. Immunopathol. 13:121–140; 1986.
Morrison, W. I.; Goddeeris, B. M.; Teale, A. J. Bovine cytotoxic T cell clones which recognize lymphoblasts infected with two antigenetically different stocks of the protozoan parasite Theileria parva. Eur. J. Immunol. 17:1703–1709; 1987.
Morrison, W. I.; Goddeeris, B. M.; Teale, A. J., et al. Cytotoxic T cells elicited in cattle challenged withTheileria parva (Muguga): evidence for restriction by class I MHC determinants and parasite strain specificfity. Parasite Immunol. 9:563–578; 1987.
Morrison, W. I.; Lalor, P. A.; Goddeeris, B. M., et al. Theileriosis: antigens and host-parasite interactions. In: Pearson, T. W., ed. Parasite antigens: towards new strategies for vaccines. New York and Basel: Marcel Dekker, Inc.; 1986:167–213.
Radley, D. E.; Brown, C. G. D. Cunningham, M. P., et al. East Coast fever: 3. Chemoprophylactic immunization of cattle using oxytetracycline and a combination of theilerial strains. Vet. Parasitol. 1:51–60; 1975.
Spooner, R. L.; Pinder, M. Monoclonal antibodies to bovine MHC products. Vet. Immunol. Immunopathol. 4:453–458; 1983.
Teale, A. J.; Kemp, S. J.; Young, F., et al. Selection by major histocompatibility type (BoLA), of lymphoid cells derived from a bovine chimaera and transformed byTheileria parasites. Parasite Immunol. 5:329–336; 1983.
Teale, A. J.; Morrison, W. I.; Goddeeris, B. M., et al. Bovine alloreactive cytotoxic cells generated in vitro: target specificity in relation to BoLA phenotype. Immunology 55:355–362; 1985.
Teillaud, J. L; Crevat, D.; Chardon, P., et al. Monoclonal antibodies as a tool for phylogenetic studies of major histocompatibility antigens and beta 2-microglobulin. Immunogenetics 15:377–384; 1982.
Vitiello, A.; Sherman, L. A. Recognition of influenza-infected cells by cytolytic T lymphocyte clones: determinant selection by class I restriction elements. J. Immunol. 131:1635–1640; 1983.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Goddeeris, B.M., Morrison, W.I. Techniques for the generation, cloning, and characterization of bovine cytotoxic T cells specific for the protozoan Theileria parva. Journal of Tissue Culture Methods 11, 101–110 (1988). https://doi.org/10.1007/BF01404140
Issue Date:
DOI: https://doi.org/10.1007/BF01404140
Key words
- cytotoxic T cell
- bovine
- protozoa
- MHC
- cloning
- Theileria parva