Advertisement

Protoplasma

, Volume 174, Issue 1–2, pp 79–81 | Cite as

N-Acetylchitooligosaccharides, biotic elicitor for phytoalexin production, induce transient membrane depolarization in suspension-cultured rice cells

  • K. Kuchitsu
  • M. Kikuyama
  • N. Shibuya
Rapid Communication

Summary

N-acetylchitooligosaccharides (fragments of chitin) elicit the production of phytoalexin in suspension-cultured rice cells. This oligosaccharide elicitor induced rapid and transient membrane depolarization at sub-nanomolar concentrations. Only the oligomers with a certain degree of polymerization were active, while deacetylated chitooligosaccharides caused no effect. Such specificity coincided well with that for the elicitor activity, suggesting possible involvement of this transient change in membrane potential as one of the initial signals in the signal transduction sequence for the activation of defense responses.

Keywords

Chitin fragments Elicitor Membrane potential N-Acetylchitooligosaccharides Rice suspension culture Signal transduction 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ehrhardt DW, Atkinson EM, Long SR (1992) Depolarization of alfalfa root hair membrane potential byRhizobium meliloti nod factors. Science 256: 998–1000PubMedGoogle Scholar
  2. Katou K, Tomiyama K, Okamoto H (1982) Effects of hyphal wall components ofPhytophthora infestans on membrane potential of potato tuber cells. Physiol Plant Pathol 21: 311–317Google Scholar
  3. Mathieu Y, Kurkdjian A, Xia H, Guern J, Koller A, Spiro MD, O'Neill M, Albersheim P, Darvill A (1991) Membrane responses induced by oligogalacturonides in suspension-cultured tobacco cells. Plant J 1: 333–343Google Scholar
  4. Mayer MG, Ziegler E (1988) An elicitor fromPhytophthora megasperma f. sp.glycinea influences the membrane potential of soybean cotyledonary cells. Physiol Mol Plant Pathol 33: 397–407Google Scholar
  5. Pelissier B, Thibaud JB, Grignon C, Esquerre-Tugaye MT (1986) Cell surfaces in plant-microorganism interactions. VII. Elicitor preparations from two fungal pathogens depolarize plant membranes. Plant Sci 46: 103–109Google Scholar
  6. Ryan CA, Farmer EE (1991) Oligosaccharide signals in plants: a current assessment. Annu Rev Plant Physiol Mol Biol 42: 651–674Google Scholar
  7. Thain JF, Doherty HM, Bowles DJ, Wildon DC (1990) Oligosaccharides that induce proteinase inhibitor activity in tomato plants cause depolarization of tomato leaf cells. Plant Cell Environ 13: 569–574Google Scholar
  8. Tomiyama K, Okamoto H, Katou K (1983) Effect of infection byPhytophthora infestans on the membrane potential of potato cells. Physiol Plant Pathol 22: 233–243Google Scholar
  9. Yamada A, Shibuya N, Kodama O, Akatsuka T (1993) Induction of phytoalexin formation in suspension-cultured rice cells byN-acetylchitooligosaccharides. Biosci Biotech Biochem 57: 405–409Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • K. Kuchitsu
    • 2
  • M. Kikuyama
    • 1
  • N. Shibuya
    • 2
  1. 1.Biological LaboratoryThe University of the AirChiba
  2. 2.Department of Cell BiologyNational Institute of Agrobiological ResourcesIbarakiJapan

Personalised recommendations