, Volume 174, Issue 1–2, pp 79–81 | Cite as

N-Acetylchitooligosaccharides, biotic elicitor for phytoalexin production, induce transient membrane depolarization in suspension-cultured rice cells

  • K. Kuchitsu
  • M. Kikuyama
  • N. Shibuya
Rapid Communication


N-acetylchitooligosaccharides (fragments of chitin) elicit the production of phytoalexin in suspension-cultured rice cells. This oligosaccharide elicitor induced rapid and transient membrane depolarization at sub-nanomolar concentrations. Only the oligomers with a certain degree of polymerization were active, while deacetylated chitooligosaccharides caused no effect. Such specificity coincided well with that for the elicitor activity, suggesting possible involvement of this transient change in membrane potential as one of the initial signals in the signal transduction sequence for the activation of defense responses.


Chitin fragments Elicitor Membrane potential N-Acetylchitooligosaccharides Rice suspension culture Signal transduction 


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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • K. Kuchitsu
    • 2
  • M. Kikuyama
    • 1
  • N. Shibuya
    • 2
  1. 1.Biological LaboratoryThe University of the AirChiba
  2. 2.Department of Cell BiologyNational Institute of Agrobiological ResourcesIbarakiJapan

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