Summary
Membranes and membrane proteins from undifferentiated cells and torpedo-stage embryos were compared. A comparison of marker enzyme profiles on linear sucrose gradients showed that the membrane vesicles obtained from 14-day-old embryos were consistently less dense than those obtained from undifferentiated carrot cells. The density of the endoplasmic reticulum, for instance, was 1.10g/cm3 in embryos and 1.12g/cm2 in undifferentiated cells. Proteins and glycoproteins from endoplasmic reticulum-, Golgi apparatus-, and plasma membrane-enriched fractions were compared from undifferentiated carrot cells with 14-day-old embryos by 2D SDS-PAGE. When these two tissues were compared, extensive qualitative and quantitative changes in the steady-state endomembrane and plasma membrane proteins were observed. The plasma membrane was examined further by labeling the plasma membrane proteins with sulfosuccinimidylbiotin. Using this specific label, plasma membrane proteins of 54 kD, 41 kD, 16 kD, and 15 kD were found to be uniquely associated with the embryonic state. Conversely, a 70 kD protein and a 45 kD glycoprotein were found to be associated with only undifferentiated cells. These results demonstrate that proteins of the plasma membrane exhibit distinct changes as a result of somatic embryogenesis in carrot.
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Abbreviations
- conA:
-
concanavilin A
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- p:
-
protein
- gp:
-
glycoprotein
- kD:
-
kilodalton
- SDS-PAGE:
-
sodium dodecylsulfate-polyacrylamide gel electrophoresis
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Slay, R.M., Grimes, H.D. & Hodges, T.K. Plasma membrane proteins associated with undifferentiated and embryonicDaucus carota tissue. Protoplasma 150, 139–149 (1989). https://doi.org/10.1007/BF01403670
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DOI: https://doi.org/10.1007/BF01403670